Matsuoka Shunsuke, Sugiyama Yoriko, Tateno Ryunosuke, Imamura Shihomi, Kawaguchi Eri, Osono Takashi
Graduate School of Simulation Studies, University of Hyogo 7-1-28 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan.
R Soc Open Sci. 2020 Feb 19;7(2):191952. doi: 10.1098/rsos.191952. eCollection 2020 Feb.
Community compositions of ectomycorrhizal (ECM) fungi are similar within the same host taxa. However, careful interpretation is required to determine whether the combination of ECM fungi and plants is explained by the host preference for ECM fungi, or by the influence of neighbouring heterospecific hosts. In the present study, we aimed to evaluate the effects of host species on the ECM community compositions in a forested landscape (approx. 10 km) where monodominant forest stands of six ECM host species belonging to three families were patchily distributed. A total of 180 ECM operational taxonomic units (OTUs) were detected with DNA metabarcoding. Quantitative multivariate analyses revealed that the ECM community compositions were primarily structured by host species and families, regardless of the soil environments and spatial arrangements of the sampling plots. In addition, 38 ECM OTUs were only detected from particular host tree species. Furthermore, the neighbouring plots harboured similar fungal compositions, although the host species were different. The relative effect of the spatial factors on the ECM compositions was weaker than that of host species. Our results suggest that the host preference for ECM fungi is the primary determinant of ECM fungal compositions in the forested landscape.
外生菌根（ECM）真菌的群落组成在同一宿主类群中相似。然而，需要谨慎解读才能确定ECM真菌与植物的组合是由宿主对ECM真菌的偏好，还是由相邻异种宿主的影响所解释。在本研究中，我们旨在评估宿主物种对森林景观（约10公里）中外生菌根群落组成的影响，在该景观中，属于三个科的六种外生菌根宿主物种的单优势林分呈斑块状分布。通过DNA宏条形码技术共检测到180个外生菌根操作分类单元（OTU）。定量多变量分析表明，外生菌根群落组成主要由宿主物种和科构建，而与采样地的土壤环境和空间布局无关。此外，仅在特定宿主树种中检测到38个外生菌根OTU。此外，尽管宿主物种不同，但相邻样地具有相似的真菌组成。空间因素对外生菌根组成的相对影响弱于宿主物种。我们的结果表明，宿主对ECM真菌的偏好是森林景观中外生菌根真菌组成的主要决定因素。
