Obase Keisuke, Yamanaka Satoshi, Kinoshita Akihiko, Tamai Yutaka, Yamanaka Takashi
a Microbial Ecology Laboratory, Department of Mushroom Science and Forest Microbiology, Forestry and Forest Products Research Institute.
b Hokkaido Research Center, Forestry and Forest Products Research Institute.
Mycoscience. 2021 Mar 20;62(2):124-131. doi: 10.47371/mycosci.2020.12.001. eCollection 2021.
Pure cultures of were isolated from ectomycorrhizal root tips in plantations in Hokkaido, Japan. Their phylogenetic relationships as well as vegetative hyphal characteristics on culture media were reported. Phylogenetic analysis based on the internal transcribed spacer within ribosomal DNA settled well-supported eight lineages within , and clades in . Three and one lineages were grouped with undescribed species of clade in Japan and that of the group in China, respectively. Two lineages were closely associated to but distinct from an undescribed species of clade in Japan. One lineage did not group with any sequences in the International Nucleotide Sequence Database (INSD), proposing a new taxon in the group. One lineage was grouped with in clade. All strains in each lineage displayed yellowish white, thin, filamentous colonies on Melin-Norkrans agar medium. Various differences in morphological characteristics of hyphae on pure cultures of various strains were noted, but they were frequently uncommon among strains of the same taxa. Isolation from ectomycorrhizal root tips can be among the effective ways to acquire pure cultures of strains.
从日本北海道人工林中的外生菌根根尖分离出了纯培养物。报道了它们的系统发育关系以及在培养基上的营养菌丝特征。基于核糖体DNA内转录间隔区的系统发育分析在……内确定了得到充分支持的八个谱系，以及在……内的……分支。三个和一个谱系分别与日本未描述的……分支物种和中国……组的物种归为一类。两个谱系与日本未描述的……分支物种密切相关但又有所不同。一个谱系在国际核苷酸序列数据库（INSD）中未与任何序列归为一类，这表明在……组中有一个新的分类单元。一个谱系与……分支中的……归为一类。每个谱系中的所有菌株在梅林 - 诺克兰斯琼脂培养基上均呈现黄白色、薄的丝状菌落。注意到不同菌株纯培养物中菌丝形态特征存在各种差异，但在同一分类群的菌株中这些差异通常并不常见。从外生菌根根尖分离可能是获取……菌株纯培养物的有效方法之一。
