Department of Biochemistry, University of Malakand, Chakdara Dir Lower, 18800 KPK, Pakistan.
Medicinal, Aromatic and Poisonous Plants Research Centre (MAPRC), College of Pharmacy, King Saud University, PO box 2457, Riyadh 11451, Saudi Arabia.
Biomed Res Int. 2020 Mar 19;2020:8216435. doi: 10.1155/2020/8216435. eCollection 2020.
In this study, an attempt has been made to devise a method for a large-scale production of quercetin from a medicinal plant. The natural products are first isolated from plants and then synthesized commercially. During their synthesis, a number of impurities or side products are also formed, most of which are carcinogenic. Plant products have limited side effects. Therefore, they are considered safe to be used for systemic uses. In the fruit, the ethyl acetate extract was loaded to 50 optimized silica gel columns. The effluents of columns were passed through the membrane system for concentration. A 100% recovery was achieved from the drain pipe in case of reverse osmosis membrane when the specified rely of the pilot plant was set on 25% rejection. About 95% recovery was achieved through the NF membrane while the 5% loss in permeate was recovered through magnetic carbon nanocomposite (characterized through a bar magnet, SEM, XRD, and EDX). The equilibrium time of adsorption was 83 min and followed by pseudo-first-order kinetics. The adsorption equilibrium data fitted well to the Langmuir isotherm model. Through the devised method, quercetin was successfully concentrated with high efficiencies; however, further studies are needed to validate the method.
在这项研究中，我们试图设计一种从药用植物中大规模生产槲皮素的方法。天然产物首先从植物中分离出来，然后进行商业合成。在合成过程中，也会形成许多杂质或副产物，其中大多数是致癌的。植物产品的副作用有限。因此，它们被认为是安全的，可以用于全身使用。在果实中，将乙酸乙酯提取物加载到 50 根优化的硅胶柱上。通过膜系统对柱的流出物进行浓缩。当指定的工厂试验台的排斥率为 25%时，反渗透膜从排水管中获得了 100%的回收率。通过 NF 膜可实现约 95%的回收率，而 5%的透过液损失则通过磁性碳纳米复合材料（通过条形磁铁、SEM、XRD 和 EDX 进行表征）回收。吸附平衡时间为 83 分钟，随后遵循拟一级动力学。吸附平衡数据很好地符合朗缪尔等温线模型。通过所设计的方法，可以高效地浓缩槲皮素；然而，需要进一步的研究来验证该方法。
