Bulmer J N, Morrison L, Johnson P M
Department of Pathology, University of Leeds, U.K.
J Reprod Immunol. 1988 Dec;14(3):291-302. doi: 10.1016/0165-0378(88)90028-9.
Immunohistochemical techniques were used to investigate the expression of proliferation markers (Ki67 and transferrin receptor) by fetal trophoblast in normal human pregnancy. In placental villous tissue, transferrin receptor was detected not only on the apical syncytiotrophoblastic membrane but also on the proximal portion of cytotrophoblast columns, an area of high cellular proliferative activity. The majority of cells in cytotrophoblast columns and shell showed nuclear reactivity with Ki67. Villous syncytiotrophoblast was uniformly unreactive with Ki67 but a proportion of the underlying cytotrophoblast was Ki67-positive throughout pregnancy. Occasional Ki67-positive trophoblast cells were identified within chorion laeve at term. In contrast, interstitial and endovascular extravillous trophoblast in maternal uterine decidual tissue failed to label with either proliferation marker. Thus, chorionic villous cytotrophoblast and extravillous trophoblast in the chorion laeve appear to retain their proliferative capacity into late pregnancy. Cytotrophoblast columns represent a zone of cellular proliferation which may be dependent on transferrin.
采用免疫组织化学技术研究正常人类妊娠中胎儿滋养层细胞增殖标志物（Ki67和转铁蛋白受体）的表达。在胎盘绒毛组织中，转铁蛋白受体不仅在顶端合体滋养层细胞膜上被检测到，而且在细胞滋养层柱的近端部分也被检测到，这是一个细胞增殖活性高的区域。细胞滋养层柱和绒毛外滋养层壳中的大多数细胞显示出与Ki67的核反应性。绒毛合体滋养层对Ki67始终无反应，但在整个妊娠期间，部分底层细胞滋养层呈Ki67阳性。足月时，在平滑绒毛膜内偶尔可发现Ki67阳性的滋养层细胞。相比之下，母体子宫蜕膜组织中的间质和血管内绒毛外滋养层未能用任何一种增殖标志物标记。因此，绒毛膜绒毛细胞滋养层和平滑绒毛膜中的绒毛外滋养层似乎在妊娠晚期仍保留其增殖能力。细胞滋养层柱代表一个细胞增殖区域，其可能依赖于转铁蛋白。
