Abinandan Sudharsanam, Subashchandrabose Suresh R, Venkateswarlu Kadiyala, Megharaj Mallavarapu
Global Centre for Environmental Remediation (GCER), Faculty of Science, University of Newcastle, ATC Building, Callaghan, New South Wales 2308, Australia.
Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), University of Newcastle, ATC Building, Callaghan, New South Wales 2308, Australia.
ACS Omega. 2020 Mar 19;5(12):6888-6894. doi: 10.1021/acsomega.0c00255. eCollection 2020 Mar 31.
Sustainable resource recovery is the key to manage the overburden of various waste entities of mining practices. The present study demonstrates for the first time a novel approach for iron recovery and biodiesel yield from two acid-adapted microalgae, sp. MAS1 and sp. MAS3, grown in synthetic acid mine drainage (SAMD). Virtually, there was no difference in the growth of the strain MAS3 both in Bold's basal medium (control) and SAMD. Using the IC level (200 mg L) and a lower concentration (50 mg L) of iron in SAMD, the cell granularity, exopolysaccharide (EPS) secretion, iron recovery, and biodiesel were assessed in both the strains. Both cell granularity and accumulation of EPS were significantly altered under metal stress in SAMD, resulting in an increase in total accumulation of iron. Growth of the microalgal strains in SAMD yielded 12-20% biodiesel, with no traces of heavy metals, from the biomass. The entire amount of iron, accumulated intracellularly, was recovered in the residual biomass. Our results on the ability of the acid-adapted microalgal strains in iron recovery and yield of biodiesel when grown in SAMD indicate that they could be the potential candidates for use in bioremediation of extreme habitats like AMD.
可持续资源回收是管理采矿作业中各种废物实体负担过重问题的关键。本研究首次展示了一种从两种适应酸性环境的微藻（MAS1菌株和MAS3菌株）中回收铁和生产生物柴油的新方法，这两种微藻生长在合成酸性矿山排水（SAMD）中。实际上，在Bold基础培养基（对照）和SAMD中，MAS3菌株的生长没有差异。使用SAMD中IC水平（200 mg/L）和较低浓度（50 mg/L）的铁，对两种菌株的细胞粒度、胞外多糖（EPS）分泌、铁回收和生物柴油进行了评估。在SAMD中的金属胁迫下，细胞粒度和EPS积累均发生显著变化，导致铁的总积累量增加。微藻菌株在SAMD中的生长从生物质中产生了12%-20%的生物柴油，且无重金属痕迹。细胞内积累的全部铁在残余生物质中被回收。我们关于适应酸性环境的微藻菌株在SAMD中生长时回收铁和生产生物柴油能力的结果表明，它们可能是用于酸性矿山排水等极端生境生物修复的潜在候选者。
