Gómez-Machuca Horacio, Quiroga-Campano Cinthia, Zapata-Torres Gerald, Jullian Carolina
Departamento de Química Orgánica y Fisicoquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Olivos 1007, Independencia, Santiago 8380492, Chile.
Departamento de Química Inorgánica y Analítica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Olivos 1007, Independencia, Santiago 8380492, Chile.
ACS Omega. 2020 Mar 20;5(12):6928-6936. doi: 10.1021/acsomega.0c00275. eCollection 2020 Mar 31.
The interaction mechanism between a scarcely soluble copper(II) complex of Cu(II)-6-hydroxychromone-3-carbaldehyde-(3'-hydroxy)benzoylhydrazone () in aqueous solution and its DMβCD complex was studied in the presence of DNA through spectroscopy and thermodynamic methods. The thermodynamic results indicate that the binding process of the -DMβCD inclusion complex is a spontaneous process and the inclusion is enthalpy-driven. The binding constants of and -DMβCD with DNA are 2.69 × 10 and 14.7 × 10 L mol, respectively. The stoichiometry of the complex is 1:1, and the determined thermodynamic indicates that the process of binding is spontaneous and entropy-driven. A competitive binding titration with ethidium bromide revealed that efficiently displaces EB from the EB-DNA system. In addition to the thermal denaturation experiments and docking studies, we can confirm that the mode of binding of this complex to DNA is intercalation mode. The presence of DMβCD enhances the aqueous solubility of ; nevertheless, the cyclodextrin did not affect the interaction of with DNA because the inclusion complex breaks down when it binds with DNA.
通过光谱法和热力学方法，研究了难溶性铜（II）配合物Cu（II）-6-羟基色酮-3-甲醛-（3'-羟基）苯甲酰腙（）在水溶液中及其与DMβCD配合物在DNA存在下的相互作用机制。热力学结果表明，-DMβCD包合物的结合过程是一个自发过程，且该包合作用是由焓驱动的。与DNA的结合常数分别为2.69×10和14.7×10 L mol。该配合物的化学计量比为1:1，所测定的热力学结果表明结合过程是自发的且由熵驱动。用溴化乙锭进行的竞争性结合滴定表明，能有效地将EB从EB-DNA体系中置换出来。除了热变性实验和对接研究外，我们可以确认该配合物与DNA的结合模式为嵌入模式。DMβCD的存在提高了的水溶性；然而，环糊精并不影响与DNA的相互作用，因为包合物在与DNA结合时会分解。
