Department of Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, California 93106, USA.
Analyst. 2020 May 7;145(9):3440-3446. doi: 10.1039/c9an02568e. Epub 2020 Apr 7.
Fentanyl and its analogs have been at the center of the opioid epidemic currently wreaking havoc in the United States. One major element in the opioid crisis is the growing number of clandestine fentanyl labs being reported by enforcement agencies. The development of new analytical methods for detecting and identifying fentanyl and its congeners is among the useful tools in our goal to limit the use of this dangerous family of narcotics. Herein we describe an analytical technique using surface-enhanced Raman spectroscopy (SERS) and a microfluidic device, for detecting fentanyl and two of its chemical precursors, despropionylfentanyl (4ANPP) and N-phenethyl-4-piperidinone (NPP). The vibrational spectra of this family of analytes are very similar, making them difficult to distinguish by traditional means. In addition to taking advantage of the sensitivity provided by SERS, we developed a chemometric approach utilizing a hierarchical partial least squares-discriminant analysis algorithm that allowed us to distinguish spectra that possess many similar features.
芬太尼及其类似物一直是当前美国阿片类药物泛滥的核心问题。阿片类药物危机的一个主要因素是执法机构报告的越来越多的秘密芬太尼实验室。开发用于检测和识别芬太尼及其同系物的新分析方法是我们限制使用这种危险的麻醉品家族的有用工具之一。在这里，我们描述了一种使用表面增强拉曼光谱（SERS）和微流控器件的分析技术，用于检测芬太尼及其两种化学前体，去丙酰芬太尼（4ANPP）和 N-苯乙基-4-哌啶酮（NPP）。这个分析物家族的振动光谱非常相似，使得它们很难通过传统手段区分。除了利用 SERS 提供的灵敏度之外，我们还开发了一种化学计量学方法，利用层次偏最小二乘判别分析算法，使我们能够区分具有许多相似特征的光谱。
