Appl Opt. 2021 Mar 10;60(8):2354-2361. doi: 10.1364/AO.418579.
In recognition of the misuse risks of fentanyl, there is an urgent need to develop a useful and rapid analytical method to detect and monitor the opioid drug. The surface-enhanced shifted excitation Raman difference spectroscopy (SE-SERDS) method has been demonstrated to suppress background interference and enhance Raman signals. In this study, the SE-SERDS method was used for trace detection of fentanyl in beverages. To prepare the simulated illegal drug-beverages, fentanyls were dissolved into distilled water or Mizone as a series of test samples. Based on our previous work, the surface-enhanced Raman spectroscopy detection was performed on the beverages containing fentanyl by the prepared AgNPs and the SE-SERDS spectra of test samples were collected by the dual-wavelength rapid excitation Raman difference spectroscopy system. In addition, the quantitative relationship between fentanyl concentrations and the Raman peaks was constructed by the Langmuir equation. The experimental results show that the limits of quantitation for fentanyl in distilled water and Mizone were 10 ng/mL and 200 ng/mL, respectively; the correlation coefficients for the nonlinear regression were as high as 0.9802 and 0.9794, respectively; and the relative standard deviation was less than 15%. Hence, the SE-SERDS method will be a promising method for the trace analyses of food safety and forensics.
鉴于芬太尼的滥用风险，我们迫切需要开发一种有用且快速的分析方法来检测和监测阿片类药物。表面增强 shifted excitation Raman 差分光谱（SE-SERDS）方法已被证明可以抑制背景干扰并增强拉曼信号。在这项研究中，SE-SERDS 方法被用于检测饮料中的痕量芬太尼。为了制备模拟的非法药物-饮料，将芬太尼溶解在蒸馏水中或 Mizone 中作为一系列测试样品。基于我们之前的工作，通过制备的 AgNPs 对含有芬太尼的饮料进行表面增强拉曼光谱检测，并通过双波长快速激发拉曼差分光谱系统采集测试样品的 SE-SERDS 光谱。此外，通过朗缪尔方程构建了芬太尼浓度与拉曼峰之间的定量关系。实验结果表明，芬太尼在蒸馏水中和 Mizone 中的定量限分别为 10ng/mL 和 200ng/mL；非线性回归的相关系数高达 0.9802 和 0.9794；相对标准偏差小于 15%。因此，SE-SERDS 方法将是食品安全和法医学痕量分析的一种很有前途的方法。
