Kaur Ramandeep, Kaur Ripneel, Grover Aman, Rani Susheela, Malik Ashok Kumar, Kabir Abuzar, Furton Kenneth G
Department of Chemistry, Punjabi University, Patiala, Punjab, India.
Department of Chemistry, GSSDGS Khalsa College, Patiala, Punjab, India.
J Sep Sci. 2020 Jul;43(13):2626-2635. doi: 10.1002/jssc.201900978. Epub 2020 May 4.
A simple, fast, and sensitive analytical protocol using fabric-phase sorptive extraction followed by high performance liquid chromatography with ultraviolet detection has been developed and validated for the extraction of five parabens including methylparaben, ethylparaben, propylparaben, butylparaben, and benzylparaben. In the present work, sol-gel polyethylene glycol coated fabric-phase sorptive extraction membrane is used for the preconcentration of parabens (polar) from complex matrices. The use of fabric-phase sorptive extraction membrane provides a high surface area which offers high sorbent loading, shortened equilibrium time, and overall decrease in the sample preparation time. Various factors affecting the performance of fabric-phase sorptive extraction, including extraction time, eluting solvent, elution time, and pH of the sample matrix, were optimized. Separation was performed using a mobile phase consisting of water:acetonitrile (63:37; v/v) at an isocratic elution mode at a flow rate of 0.9 mL/min with wavelength at 254 nm. The calibration curves of the target analytes were prepared with good correlation coefficient values (r > 0.9955). The limit of detection values range from 0.252 to 0.580 ng/mL. Finally, the method was successfully applied to various cosmetics and personal care product samples such as rose water, deodorant, hair serum, and cream with extraction recoveries ranged between 88 and 122% with relative standard deviation <5%.
已开发并验证了一种简单、快速且灵敏的分析方法，该方法采用织物相吸附萃取，随后进行带紫外检测的高效液相色谱法，用于萃取包括对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯和对羟基苯甲酸苄酯在内的五种对羟基苯甲酸酯。在本研究中，溶胶 - 凝胶聚乙二醇涂层织物相吸附萃取膜用于从复杂基质中预富集对羟基苯甲酸酯（极性物质）。织物相吸附萃取膜的使用提供了高表面积，从而实现了高吸附剂负载量、缩短了平衡时间并总体减少了样品制备时间。对影响织物相吸附萃取性能的各种因素，包括萃取时间、洗脱溶剂、洗脱时间和样品基质的pH值进行了优化。分离采用由水：乙腈（63:37；v/v）组成的流动相，在等度洗脱模式下，流速为0.9 mL/min，波长为254 nm。目标分析物的校准曲线具有良好的相关系数值（r > 0.9955）。检测限范围为0.252至0.580 ng/mL。最后，该方法成功应用于各种化妆品和个人护理产品样品，如玫瑰水、除臭剂、护发精华液和面霜，萃取回收率在88%至122%之间，相对标准偏差<5%。
