Laboratory of Pharmaceutical Analysis, Division of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
Molecules. 2021 Mar 11;26(6):1526. doi: 10.3390/molecules26061526.
Parabens have been widely employed as preservatives since the 1920s for extending the shelf life of foodstuffs, medicines, and daily care products. Given the fact that there are some legitimate concerns related to their potential multiple endocrine-disrupting properties, the development of novel bioanalytical methods for their biomonitoring is crucial. In this study, a fabric phase sorptive extraction reversed-phase liquid chromatography method coupled with UV detection (FPSE-HPLC-UV) was developed and validated for the quantitation of seven parabens in human plasma samples. Chromatographic separation of the seven parabens and -hydroxybenzoic acid was achieved on a semi-micro Spherisorb ODS1 analytical column under isocratic elution using a mobile phase containing 0.1% (/) formic acid and 66% 49 mM ammonium formate aqueous solution in acetonitrile at flow rate 0.25 mL min with a 24-min run time for each sample. The method was linear at a concentration range of 20 to 500 ng mL for the seven parabens under study in human plasma samples. The efficiency of the method was proven with the analysis of 20 human plasma samples collected from women subjected to breast cancer surgery and to reconstructive and aesthetic breast surgery. The highest quantitation rates in human plasma samples from cancerous cases were found for methylparaben and isobutylparaben with average plasma concentrations at 77 and 112.5 ng mL. The high concentration levels detected agree with previous findings for some of the parabens and emphasize the need for further epidemiological research on the possible health effects of the use of these compounds.
自 20 世纪 20 年代以来，由于可以延长食品、药品和日常护理产品的保质期，所以人们广泛使用对羟基苯甲酸酯作为防腐剂。考虑到它们具有潜在的多种内分泌干扰特性，因此开发用于监测其生物浓度的新型生物分析方法至关重要。在这项研究中，开发并验证了一种基于纤维增强相固相萃取反相高效液相色谱-紫外检测（FPSE-HPLC-UV）的方法，用于测定人血浆样品中的七种对羟基苯甲酸酯。在等度洗脱条件下，使用含有 0.1%（/）甲酸和 66% 49 mM 甲酸铵水溶液的流动相，在流速为 0.25 mL min 的条件下，在半微 Spherisorb ODS1 分析柱上实现了七种对羟基苯甲酸酯和 -羟基苯甲酸的色谱分离，每个样品的运行时间为 24 分钟。在研究的人血浆样品中，七种对羟基苯甲酸酯的浓度范围为 20 至 500ng mL 时，方法呈线性。通过对 20 名接受乳腺癌手术和重建及美容乳房手术的女性采集的人血浆样本进行分析，证明了该方法的效率。在癌症病例的人血浆样本中，发现甲基对羟基苯甲酸酯和异丁基对羟基苯甲酸酯的定量率最高，平均血浆浓度分别为 77 和 112.5ng mL。检测到的高浓度水平与先前对某些对羟基苯甲酸酯的发现一致，强调需要对这些化合物的使用可能对健康造成的影响进行进一步的流行病学研究。
