Key Laboratory of Genetics and Breeding, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313000, China.
Key Laboratory of Genetics and Breeding, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313000, China.
Gene Expr Patterns. 2020 Jun;36:119112. doi: 10.1016/j.gep.2020.119112. Epub 2020 Apr 4.
Sox protein family is characterized by the presence of the conserved high-mobility group (HMG) box. Sox transcription factors are involved in diverse developmental process in animals, including sex-determination, organogenesis, embryogenesis, neurogenesis, and cell fate decision. In this study, 23 Sox genes were identified based on the Culter alburnus whole-genome sequence and categorized into six subfamilies according to the conserved HMG-box domain. The duplicates of four members revealed that Sox genes in the teleost fishes underwent significant expansion. Moreover, their expression pattern in gonad tissues were analyzed by RNA-seq and qRT-PCR, and Sox9b was determined as a key gene that was essential for testis development. This current study will provide new insight into the role of Sox gene family in fish sex determination and differentiation.
Sox 蛋白家族的特征是存在保守的高迁移率族（HMG）盒。Sox 转录因子参与动物的多种发育过程，包括性别决定、器官发生、胚胎发生、神经发生和细胞命运决定。在这项研究中，根据 Culter alburnus 全基因组序列鉴定了 23 个 Sox 基因，并根据保守的 HMG 盒结构域将其分为六个亚家族。四个成员的重复表明，硬骨鱼类中的 Sox 基因经历了显著的扩张。此外，通过 RNA-seq 和 qRT-PCR 分析了它们在性腺组织中的表达模式，确定 Sox9b 是睾丸发育所必需的关键基因。本研究将为 Sox 基因家族在鱼类性别决定和分化中的作用提供新的见解。
