Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
Department for Sustainable Food Process, Research Centre for Nutrigenomics and Proteomics, University Cattolica del Sacro Cuore, 29122 Piacenza, Italy.
Int J Mol Sci. 2020 Apr 3;21(7):2482. doi: 10.3390/ijms21072482.
Sweet basil ( L.) is a highly versatile and globally popular culinary herb, and a rich source of aromatic and bioactive compounds. Particularly for leafy vegetables, nutrient management allows a more efficient and sustainable improvement of crop yield and quality. In this work, we investigated the effects of balanced modulation of the concentration of two antagonist anions (nitrate and chlorine) in basil. Specifically, we evaluated the changes in yield and leaf metabolic profiles in response to four different NO:Cl ratios in two consecutive harvests, using a full factorial design. Our work indicated that the variation of the nitrate-chloride ratio exerts a large effect on both metabolomic profile and yield in basil, which cannot be fully explained only by an anion-anion antagonist outcome. The metabolomic reprogramming involved different biochemical classes of compounds, with distinctive traits as a function of the different nutrient ratios. Such changes involved not only a response to nutrients availability, but also to redox imbalance and oxidative stress. A network of signaling compounds, including NO and phytohormones, underlined the modeling of metabolomic signatures. Our work highlighted the potential and the magnitude of the effect of nutrient solution management in basil and provided an advancement towards understanding the metabolic response to anion antagonism in plants.
甜罗勒（L.）是一种用途广泛且在全球范围内广受欢迎的烹饪香草，也是芳香和生物活性化合物的丰富来源。特别是对于叶菜类蔬菜，通过营养管理，可以更有效地提高作物产量和质量。在这项工作中，我们研究了平衡调节两种拮抗阴离子（硝酸盐和氯）浓度对罗勒的影响。具体来说，我们使用完全析因设计，在两个连续的收获期内，评估了四种不同的 NO：Cl 比例对产量和叶片代谢谱的变化。我们的工作表明，硝酸盐-氯离子比的变化对罗勒的代谢组学特征和产量都有很大的影响，仅通过阴离子-阴离子拮抗作用的结果并不能完全解释。代谢组学的重编程涉及不同类别的化合物的生化，具有不同的特征，这取决于不同的营养比例。这些变化不仅涉及对养分供应的反应，还涉及到氧化还原失衡和氧化应激。包括一氧化氮和植物激素在内的一系列信号化合物，强调了对代谢组学特征进行建模。我们的工作强调了在罗勒中管理营养液的潜力和效果的重要性，并为理解植物中阴离子拮抗作用的代谢反应提供了进展。
