Barbosa Ana, Araújo Daniela, Ribeiro Eduarda, Henriques Mariana, Silva Sónia
LIBRO-Laboratório de Investigação em Biofilmes Rosário Oliveira, CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Microorganisms. 2020 Apr 3;8(4):511. doi: 10.3390/microorganisms8040511.
remains the most problematic of all species, causing severe infections. Adaptation to different human body niches, such oral and urinary tracts, has been shown to be essential for survival and critical for virulence of Thus, the present work aimed to study the behaviour of on simulated human body fluids (artificial saliva and urine) at different values of pH (pH 5.8 and 7) by determining its ability to develop two of the most important virulence factors: biofilms and filamentous forms. Under this study, it was demonstrated that was able to grow as free cells and to develop biofilm communities composed of multiple cell types (yeast and elongated hyphal cells) on both simulated human body fluids and under different pH. It was interesting to note that the pH had little impact on planktonic and biofilm growth, despite influencing the development of filamentous shapes in artificial saliva and urine. So, it was possible to infer that presents a high plasticity and adaptability to different human body fluids, namely saliva and urine. These can be the justification for the high number of oral and urinary candidiasis in the whole world.
仍然是所有物种中问题最大的，会引发严重感染。已证明适应人体不同部位，如口腔和尿道，对其生存至关重要，对其毒力也至关重要。因此，本研究旨在通过测定其形成两种最重要毒力因子（生物膜和丝状形态）的能力，来研究其在不同pH值（pH 5.8和7）的模拟人体体液（人工唾液和尿液）中的行为。在这项研究中，证明了它能够以游离细胞形式生长，并在模拟人体体液和不同pH条件下形成由多种细胞类型（酵母和伸长的菌丝细胞）组成的生物膜群落。有趣的是，尽管pH值会影响人工唾液和尿液中丝状形态的形成，但对其浮游生长和生物膜生长影响很小。所以，可以推断它对不同的人体体液，即唾液和尿液，具有高度的可塑性和适应性。这可能是全世界口腔念珠菌病和泌尿生殖道念珠菌病高发的原因。
