短期禁欲后第二次射精上浮前后的精子参数
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence.
作者信息
Manna Claudio, Barbagallo Federica, Manzo Raffaella, Rahman Ashraf, Francomano Davide, Calogero Aldo E
机构信息
Biofertility IVF and Infertility Center, 00198 Rome, Italy.
Department of Clinical and Experimental Medicine, University of Catania, 95125, Catania, Italy.
出版信息
J Clin Med. 2020 Apr 5;9(4):1029. doi: 10.3390/jcm9041029.
BACKGROUND
Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoospermic men and oligo-astheno-teratozoospermic (OAT) patients.
MATERIAL AND METHODS
Semen analyses and swim-up preparations of two consecutive semen samples (collected within 1 h) were carried out in 30 normozoospermic and 35 OAT patients enrolled in an assisted reproductive technique (ART) program.
RESULTS
Compared to the first ejaculate, the second sample showed a higher percentage of spermatozoa with normal form ( < 0.01) and lower percentage of spermatozoa with DNA fragmentation ( < 0.01) in normozoospermic men, whereas a higher percentage of spermatozoa with progressive motility ( < 0.001) and normal morphology ( < 0.0001) was found in OAT patients. Swim-up separation showed a lower DNA fragmentation rate ( < 0.05) in the second ejaculate in normozoospermic men, whereas the second ejaculate of OAT patents showed an increase in normally-shaped spermatozoa ( < 0.01) and lower percentage of spermatozoa with fragmented DNA ( < 0.001) compared to the first one.
CONCLUSIONS
Swim-up separation of a second ejaculate collected within 1 h might be suggested for ART procedures, especially in OAT patients.
背景
近期研究证实了短时间禁欲对精子参数的有益影响。本研究旨在评估正常精子症男性和少弱畸精子症（OAT）患者在短时间禁欲后获取的第二次射精经上游法处理前后的精子活力、形态和DNA碎片率。
材料与方法
对30例正常精子症患者和35例参与辅助生殖技术（ART）项目的OAT患者进行了连续两份精液样本（1小时内采集）的精液分析和上游法处理。
结果
与第一次射精相比，正常精子症男性的第二次样本显示正常形态精子的百分比更高（<0.01），DNA碎片精子的百分比更低（<0.01）；而OAT患者中具有前向运动能力（<0.001）和正常形态（<0.0001）的精子百分比更高。上游法分离显示，正常精子症男性的第二次射精DNA碎片率更低（<0.05）；而与第一次射精相比，OAT患者的第二次射精正常形态精子数量增加（<0.01），DNA碎片精子百分比降低（<0.001）。
结论
对于ART程序，尤其是OAT患者，建议对1小时内采集的第二次射精进行上游法分离。
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