肌醇：它能改善精子线粒体功能和精子活力吗？

Myoinositol: does it improve sperm mitochondrial function and sperm motility?

机构信息

Section of Endocrinology, Andrology, and Internal Medicine, Department of Internal Medicine and Systemic Diseases, University of Catania, Catania, Italy.

出版信息

Urology. 2012 Jun;79(6):1290-5. doi: 10.1016/j.urology.2012.03.005.

DOI:10.1016/j.urology.2012.03.005

PMID:22656408

Abstract

OBJECTIVE

To evaluate whether an improvement in mitochondrial membrane potential was associated with sperm motility amelioration and greater sperm recovery after the swim-up procedure. A second purpose was to evaluate the effects of myoinositol (MYO) on sperm apoptosis, quality of chromatin compaction, and DNA integrity.

METHODS

Spermatozoa from 20 normozoospermic men and 20 patients with oligo-astheno-teratozoospermia were incubated in vitro with 2 mg/mL of MYO or phosphate-buffered saline as a control for 2 hours. After this incubation period, sperm motility was evaluated. Flow cytometry was used to analyze the mitochondrial membrane potential, phosphatidylserine externalization, chromatin compactness, and DNA fragmentation. We also evaluated the total number of motile spermatozoa recovered after swim-up after incubation with MYO or phosphate-buffered saline.

RESULTS

MYO significantly increased the percentage of spermatozoa with progressive motility in both normozoospermic men and patients with oligo-astheno-teratozoospermia. Motility improvement in the latter group was associated with a significant increase in the percentage of spermatozoa with high mitochondrial membrane potential. MYO had no effects on mitochondrial function in spermatozoa from normozoospermic men. Sperm phosphatidylserine externalization, chromatin compactness, and DNA fragmentation were unaffected by MYO in both groups. After incubation with MYO, the total number of spermatozoa recovered after swim-up had improved significantly in both groups.

CONCLUSION

These data show that MYO increases sperm motility and the number of spermatozoa retrieved after swim-up in both normozoospermic men and patients with abnormal sperm parameters. In patients with oligo-astheno-teratozoospermia, the improvement in these parameters was associated with improved sperm mitochondrial function. These findings support the use of MYO in both in vivo- and in vitro-assisted reproductive techniques.

摘要

目的

评估线粒体膜电位的改善是否与精子活力的改善以及泳道后精子回收率的提高有关。第二个目的是评估肌醇（MYO）对精子凋亡、染色质致密性和 DNA 完整性的影响。

方法

体外将 20 名正常生育力男性和 20 名少弱畸形精子症患者的精子与 2mg/ml 的 MYO 或磷酸盐缓冲盐水（对照）孵育 2 小时。孵育结束后，评估精子活力。使用流式细胞术分析线粒体膜电位、磷脂酰丝氨酸外翻、染色质致密性和 DNA 碎片化。我们还评估了孵育 MYO 或磷酸盐缓冲盐水后泳道后回收的总活力精子数。

结果

MYO 显著增加了正常生育力男性和少弱畸形精子症患者的精子前向运动比例。后者的活力改善与具有高线粒体膜电位的精子比例显著增加有关。MYO 对正常生育力男性精子的线粒体功能没有影响。在两组中，MYO 对精子的磷脂酰丝氨酸外翻、染色质致密性和 DNA 碎片化均无影响。孵育 MYO 后，两组泳道后回收的精子总数均显著增加。