Tapeinos C, Efthimiadou E K, Boukos N, Charitidis C A, Koklioti M, Kordas G
NCSR "Demokritos", Sol-Gel Laboratory, Institute for Advanced Materials, Physicochemical Properties, Nanotechnology and Microsystems, 153 10 Aghia Paraskevi Attikis, Greece.
J Mater Chem B. 2013 Jan 14;1(2):194-203. doi: 10.1039/c2tb00013j. Epub 2012 Oct 22.
A soft template method was used for the synthesis of pH-responsive microcontainers with an inner cavity. Poly(glycidyl methacrylate) (PGMA) microspheres of narrow size distribution were synthesized by soap-free radical emulsion polymerization and the coating of the microspheres was carried out by the same procedure. The procedure consists of two steps. In the first step the sacrificial template is synthesized and in the second step the shell is formed. Acrylic acid was used as a coating monomer, with the aim of introducing pH sensitivity in the synthesized microcontainers. A loading and release study of the anthracycline drug doxorubicin (DOX) was also carried out. The toxicity evaluation of the drug was carried out using the MTT assay, and the necrotic effect was studied using trypan blue.
采用软模板法合成了具有内腔的pH响应性微容器。通过无皂自由基乳液聚合合成了粒径分布窄的聚甲基丙烯酸缩水甘油酯（PGMA）微球，并采用相同的方法对微球进行包覆。该过程包括两个步骤。第一步合成牺牲模板，第二步形成壳层。使用丙烯酸作为包覆单体，目的是在合成的微容器中引入pH敏感性。还进行了蒽环类药物阿霉素（DOX）的负载和释放研究。使用MTT法进行药物的毒性评估，并使用台盼蓝研究坏死效应。
