Nakazawa A, Suzuki N, Tamada T
Antimicrob Agents Chemother. 1977 Feb;11(2):219-24. doi: 10.1128/AAC.11.2.219.
A rapid rate and large amounts of colicin E1 synthesis were induced by incubating colicinogenic cells without aeration in the presence of glucose. The decrease in ability of colicin induction by aeration was not due to an ample supply of oxygen, but to the simple agitation of the bacterial suspension. Sugars other than glucose that support colicin synthesis were mannose, fructose, glucose-6-phosphate, and mannitol. The effect of sugars in mutants deficient in enzymes of glucose metabolism suggested that some intermediary metabolites between glucose and pyruvate are closely related to the stimulation of colicin synthesis under these conditions. Analyses of deoxyribonucleic acid replication indicated that the induced replication of the colicin E1 plasmid deoxyribonucleic acid occurred even under conditions in which colicin was not actively synthesized.
通过在葡萄糖存在的情况下对产大肠杆菌素的细胞进行不曝气培养，可诱导大肠杆菌素E1快速且大量地合成。曝气导致大肠杆菌素诱导能力下降并非由于充足的氧气供应，而是由于细菌悬液的简单搅动。除葡萄糖外，支持大肠杆菌素合成的糖类还有甘露糖、果糖、6-磷酸葡萄糖和甘露醇。对葡萄糖代谢酶缺陷型突变体中糖类的影响表明，在这些条件下，葡萄糖和丙酮酸之间的一些中间代谢物与大肠杆菌素合成的刺激密切相关。脱氧核糖核酸复制分析表明，即使在大肠杆菌素未活跃合成的条件下，大肠杆菌素E1质粒脱氧核糖核酸的诱导复制也会发生。
