Hoo Siew Pei, Loh Qiu Li, Yue Zhilian, Fu Jing, Tan Timothy T Y, Choong Cleo, Chan Peggy P Y
Department of Chemical Engineering, Monash University, Australia.
J Mater Chem B. 2013 Jun 28;1(24):3107-3117. doi: 10.1039/c3tb00446e. Epub 2013 May 20.
This study describes the preparation and characterization of a biodegradable 3D hydrogel constructed from hydroxypropyl cellulose (HPC), modified with bifunctional methacrylic anhydride (MA) to form hydroxypropyl cellulose methacrylate (HPC-MA), for adipose tissue engineering applications. The hydrogels were prepared from three different concentrations (10 wt%, 15 wt% and 20 wt%) of HPC-MA with 0.35 degree of substitution. HPC-MA hydrogel scaffolds with open biphasic features were prepared by exploiting the thermal responsive phase behavior of HPC and temperature mediated phase separation of HPC-MA. The resulting scaffolds exhibited pore sizes ranging from 30 to 300 μm and an interconnected porosity of ∼90%. The swelling ratio (SR) and storage modulus of HPC-MA scaffolds were in the range of 12.94 to 35.83 and 0.75 to 4.28 kPa, respectively. The swelling ratio and storage modulus suggested that the scaffold exhibits high water retention, allowing medium exchange during cell culturing and that it is suitable for adipose tissue regeneration. The HPC-MA scaffolds were found to be biocompatible to human adipose-derived stem cells (ASCs). ASCs were successfully differentiated into the adipocytes inside the scaffolds, and therefore demonstrated the potential application of these HPC-MA scaffolds for adipose tissue engineering.
本研究描述了一种用于脂肪组织工程应用的可生物降解三维水凝胶的制备与表征。该水凝胶由羟丙基纤维素（HPC）构建而成，用双功能甲基丙烯酸酐（MA）进行改性以形成甲基丙烯酸羟丙酯（HPC-MA）。水凝胶由三种不同浓度（10 wt%、15 wt%和20 wt%）且取代度为0.35的HPC-MA制备而成。利用HPC的热响应相行为和HPC-MA的温度介导相分离，制备出具有开放双相特征的HPC-MA水凝胶支架。所得支架的孔径范围为30至300μm，连通孔隙率约为90%。HPC-MA支架的溶胀率（SR）和储能模量分别在12.94至35.83和0.75至4.28 kPa范围内。溶胀率和储能模量表明该支架具有高保水性，允许在细胞培养过程中进行培养基交换，并且适用于脂肪组织再生。发现HPC-MA支架对人脂肪来源干细胞（ASCs）具有生物相容性。ASCs在支架内成功分化为脂肪细胞，因此证明了这些HPC-MA支架在脂肪组织工程中的潜在应用。
