Polymer Science Group, Department of Chemical and Biomolecular Engineering, and §Department of Chemical and Biomolecular Engineering, University of Melbourne , Parkville, Melbourne, Victoria 3010, Australia.
Biomacromolecules. 2016 Sep 12;17(9):2981-91. doi: 10.1021/acs.biomac.6b00817. Epub 2016 Aug 8.
Synthetic polypeptides are a class of bioinspired polymers with well demonstrated biocompatibility, enzyme biodegradability, and cell adhesive properties, making them promising materials for the preparation of macroporous hydrogels as 3D cellular scaffolds. Three-dimensional macroporous hydrogels composed entirely of biocompatible and enzyme biodegradable synthetic polypeptides have thus been prepared. Under cryoconditions, macroporous hydrogels in the form of macroporous cryogels were prepared using a single copolymer component through direct EDC/sulfo-NHS zero-length cross-linking between poly(l-glutamic acid) (PLG) and poly(l-lysine) (PLL) residues on a PLG-r-PLL random copolypeptide chain. The resulting macroporous cryogels were found to contain large interconnected pores (≥100 μm) highly suitable for tissue engineering applications. Tuning the relative ratios of the amino acid components could result in cryogels with very different pore structures, swelling, and mechanical properties, suitable for developing gels for a range of possible soft tissue engineering applications. These cryogels were shown to be enzymatically biodegradable and demonstrated excellent biocompatibility, cell attachment and cell proliferation profiles with mammalian fibroblast (NIH-3T3) cells, demonstrating the appeal of these novel cryogels as highly suitable cellular scaffolds.
合成多肽是一类仿生聚合物，具有良好的生物相容性、酶可降解性和细胞黏附性，使其成为制备作为 3D 细胞支架的大孔水凝胶的有前途的材料。因此，已经制备了完全由生物相容和酶可降解的合成多肽组成的三维大孔水凝胶。在冷冻条件下，通过在聚（L-谷氨酸）（PLG）和聚（L-赖氨酸）（PLL）残基之间的 PLG-r-PLL 无规共多肽链上直接进行 EDC/sulfo-NHS 零长度交联，使用单一共聚物成分制备了大孔水凝胶形式的大孔冷冻凝胶。发现所得的大孔冷冻凝胶包含非常适合组织工程应用的大互连孔（≥100μm）。调节氨基酸成分的相对比例可以得到具有非常不同的孔结构、溶胀和机械性能的冷冻凝胶，适合开发一系列可能的软组织工程应用的凝胶。这些冷冻凝胶可被酶降解，并且与哺乳动物成纤维细胞（NIH-3T3）具有极好的生物相容性、细胞附着和细胞增殖特性，表明这些新型冷冻凝胶作为高度合适的细胞支架具有吸引力。
