Wei Xiaofeng, Zheng Lingyan, Luo Fang, Lin Zhenyu, Guo Longhua, Qiu Bin, Chen Guonan
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, China.
J Mater Chem B. 2013 Apr 7;1(13):1812-1817. doi: 10.1039/c3tb00501a. Epub 2013 Feb 18.
Based on the adsorption of a metal-organic framework (MOF) to DNA, a fluorescent biosensor for HN antibodies has been developed. In this system, a short oligonucleotide was modified with the fluorescent dye 5'6-FAM (5'6-carboxyfluorescein) as the fluorescent DNA probe. With the introduction of a MOF aqueous solution, the DNA probe can be adsorbed by the MOF and the fluorescence of the dye will be quenched by the MOF. Subsequently, exonuclease I (Exo I) is employed to specifically hydrolyze the DNA probe at the 3'-terminus and the fluorescent dye FAM is released from the MOF, which results in recovery of the fluorescence. If the 3'-ends of the DNA probe are linked to HN antigens, which can specifically recognize the HN antibody, the hydrolysis of Exo I would be inhibited, so the fluorescence of the system would not recover. The fluorescence is related to the logarithm of the HN antibody concentration in the range 1.0 × 10-5.0 × 10 mol L with a detection limit of 1.6 × 10 mol L (S/N = 3). The proposed method has been applied to detect the HN antibody in serum samples with satisfactory results.
基于金属有机框架（MOF）对DNA的吸附作用，开发了一种用于HN抗体的荧光生物传感器。在该系统中，一种短寡核苷酸用荧光染料5'6-FAM（5'6-羧基荧光素）修饰作为荧光DNA探针。随着MOF水溶液的引入，DNA探针可被MOF吸附，染料的荧光将被MOF淬灭。随后，使用核酸外切酶I（Exo I）特异性地在3'-末端水解DNA探针，荧光染料FAM从MOF中释放出来，导致荧光恢复。如果DNA探针的3'-末端与可特异性识别HN抗体的HN抗原相连，Exo I的水解将受到抑制，因此系统的荧光不会恢复。在1.0×10 - 5.0×10 mol/L范围内，荧光与HN抗体浓度的对数相关，检测限为1.6×10 mol/L（S/N = 3）。所提出的方法已应用于检测血清样品中的HN抗体，结果令人满意。
