Fitzgerald R L, Ramos J M, Bogema S C, Poklis A
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
J Anal Toxicol. 1988 Sep-Oct;12(5):255-9. doi: 10.1093/jat/12.5.255.
The objective of this study is to determine whether R(-)-methamphetamine inhaled from nasal inhalers produces positive methamphetamine results in currently used urine drug screening procedures and to present a rapid method for distinguishing the optical isomers of methamphetamine. Urine from three subjects inhaling from a Vicks Nasal Inhaler every 20 min for six hours tested positive for methamphetamine by EMIT, Toxilab, TDx, and GC/MS. The chiral derivatizing reagent N-trifluoroacetyl-L-prolyl chloride (L-TPC) was used to form methamphetamine diastereomers allowing rapid identification of each stereoisomer of methamphetamine present in the urine samples. Urine samples positive for amphetamines during routine drug screening were determined to consist of a racemic mixture of methamphetamine. The isomeric composition of methamphetamine present in a urine sample indicates the probable source of the drug.
本研究的目的是确定从鼻腔吸入器吸入的R(-)-甲基苯丙胺在目前使用的尿液药物筛查程序中是否会产生阳性的甲基苯丙胺检测结果，并提出一种区分甲基苯丙胺光学异构体的快速方法。三名受试者每隔20分钟从维克斯鼻腔吸入器吸入药物，持续6小时，其尿液经EMIT、Toxilab、TDx和GC/MS检测，甲基苯丙胺呈阳性。使用手性衍生试剂N-三氟乙酰-L-脯氨酰氯（L-TPC）形成甲基苯丙胺非对映异构体，从而能够快速鉴定尿液样本中存在的每种甲基苯丙胺立体异构体。在常规药物筛查中苯丙胺呈阳性的尿液样本被确定为由甲基苯丙胺的外消旋混合物组成。尿液样本中存在的甲基苯丙胺的异构体组成表明了药物的可能来源。
