Clark M, Dar M S
Department of Pharmacology, East Carolina University, School of Medicine, Greenville, NC 27834.
J Neurosci Methods. 1988 Oct;25(3):243-9. doi: 10.1016/0165-0270(88)90139-2.
The effect of acute ethanol on adenosine content in four motor areas of the male Sprague-Dawley rat brain was investigated using HPLC-fluorescence detection. Since basal adenosine levels are difficult to assess due to extremely rapid turnover of adenosine, four different methods of sacrifice were also evaluated for adenosine measurement. The rank order for best results in measuring adenosine content with the various methods of sacrifice was: focused microwave irradiation greater than decapitation into liquid nitrogen greater than immersion into liquid nitrogen greater than decapitation. These differences probably reflect differences in degree of hypoxia and postmortem anoxia, factors well known to elevate adenosine, associated with the sacrifice method. Focused microwave irradiation of appropriate duration was found to be the best method of sacrifice and the results probably most closely reflect true basal adenosine levels. No significant alteration in adenosine content in any brain region examined was observed due to ethanol administration.
采用高效液相色谱-荧光检测法，研究了急性乙醇对雄性斯普拉格-道利大鼠脑四个运动区域腺苷含量的影响。由于腺苷周转极快，难以评估其基础水平，因此还评估了四种不同的处死方法用于腺苷测量。不同处死方法测量腺苷含量的最佳结果排序为：聚焦微波辐射大于液氮断头法大于液氮浸泡法大于断头法。这些差异可能反映了与处死方法相关的缺氧和死后缺氧程度的差异，而缺氧和死后缺氧是已知会升高腺苷的因素。发现适当持续时间的聚焦微波辐射是最佳处死方法，其结果可能最接近真实的基础腺苷水平。未观察到乙醇给药导致所检测的任何脑区腺苷含量有显著变化。
