Lust W D, Passonneau J V, Veech R L
Science. 1973 Jul 20;181(4096):280-2. doi: 10.1126/science.181.4096.280.
Fixation of rat brain tissue by freeze-blowing, microwave irradiation, immersion of whole rats in liquid nitrogen, and decapitation into liquid nitrogen indicates that postmortem changes in metabolites and enzyme forms are minimal in freeze-blown brains. Cyclic adenosine monophosphate levels are lowest in microwave-irradiated brains, which has been interpreted by some investigators to indicate rapid fixation and minimal anoxia. However, the changes in phosphocreatine, adenosine triphosphate, lactate, and phosphorylase clearly demonstrate that fixation by freeze-blowing or immersion in liquid nitrogen more closely approximate the state in vivo.
通过冷冻吹气、微波辐射、将整只大鼠浸入液氮以及断头后放入液氮对大鼠脑组织进行固定，结果表明，冷冻吹制的大脑中代谢物和酶形式的死后变化最小。微波辐射的大脑中环状单磷酸腺苷水平最低，一些研究人员将此解释为表明快速固定和最小程度的缺氧。然而，磷酸肌酸、三磷酸腺苷、乳酸和磷酸化酶的变化清楚地表明，通过冷冻吹气或浸入液氮进行固定更接近体内状态。
