Jia Jingwei, Wang Zhengke, Lu Wentao, Yang Ling, Wu Qingwen, Qin Wei, Hu Qiaoling, Tang Ben Zhong
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
J Mater Chem B. 2014 Dec 21;2(47):8406-8411. doi: 10.1039/c4tb01158a. Epub 2014 Oct 29.
A novel chitosan-based fluorescent bioconjugate (TPE-CS) with aggregation-induced emission (AIE) characteristic is synthesized and used as a fluorescent probe for monitoring layer-by-layer self-assembly process of natural polyelectrolytes. QCM results and contact angle measurement indicate that this AIE active TPE-CS bioconjugate can be assembled with alginate (ALG) through layer-by-layer deposition. Ellipsometry and fluorescence (FL) spectroscopy show an exponential growth of the TPE-CS/ALG multilayer films. Moreover, the exponential relationship between the FL intensity and the number of bilayers, which is in accordance with the thickness variation of multilayer films, provides solid evidence for its capacity to monitor the layer-by-layer self-assembly process.
合成了一种具有聚集诱导发光（AIE）特性的新型壳聚糖基荧光生物共轭物（TPE-CS），并将其用作荧光探针来监测天然聚电解质的逐层自组装过程。石英晶体微天平（QCM）结果和接触角测量表明，这种具有AIE活性的TPE-CS生物共轭物可以通过逐层沉积与海藻酸盐（ALG）组装在一起。椭偏仪和荧光（FL）光谱显示TPE-CS/ALG多层膜呈指数增长。此外，FL强度与双层数之间的指数关系与多层膜的厚度变化一致，为其监测逐层自组装过程的能力提供了确凿证据。
