Wang Zhengke, Liu Yalan, Jia Jingwei, Chen Sijie, Qin Wei, Hu Qiaoling, Tang Ben Zhong
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
J Mater Chem B. 2016 Aug 21;4(31):5265-5271. doi: 10.1039/c6tb01466f. Epub 2016 Jul 22.
Fluorescent nanoparticles used to detect important biological events in living cells or animals are in increasing demand in the biological and biomedical fields, and have attracted much attention from chemists and biologists in the past decade. Here, one aggregation-induced emission (AIE) bioconjugate, tetraphenylethene labelled chitosan (TPE-CS), is synthesized, which could be strongly emissive in the solid state. TPE-CS is used as a coating agent for negatively charged hydroxyapatite (HA) nanoparticles, and TPE-CS/HA nanocomposites with positive charges are well dispersed in their aqueous solution with a diameter of 111.9 nm. MTT assay indicates that the fluorescent TPE-CS/HA nanoparticles have good cytocompatibility. 293T cells are imaged by TPE-CS/HA nanoparticles. First, the nanoparticles are adhered to the cell membrane, and then many more particles are endocytosed through phagocytotic vesicles by culturing for a long time, resulting in a much stronger fluorescence emission. TPE-CS/HA bioprobes could strongly bind the cell cytoplasmic region, and might have promising applications in tumor diagnosis, long-term cell tracing, drug metabolism and drug delivery systems.
用于检测活细胞或动物中重要生物事件的荧光纳米颗粒在生物和生物医学领域的需求日益增加，在过去十年中引起了化学家和生物学家的广泛关注。在此，合成了一种聚集诱导发光（AIE）生物共轭物，四苯乙烯标记的壳聚糖（TPE-CS），其在固态下具有强烈的发光性。TPE-CS用作带负电荷的羟基磷灰石（HA）纳米颗粒的包覆剂，带正电荷的TPE-CS/HA纳米复合材料在其水溶液中分散良好，直径为111.9nm。MTT法表明荧光TPE-CS/HA纳米颗粒具有良好的细胞相容性。用TPE-CS/HA纳米颗粒对293T细胞进行成像。首先，纳米颗粒附着在细胞膜上，然后通过长时间培养，更多的颗粒通过吞噬小泡被内吞，导致更强的荧光发射。TPE-CS/HA生物探针可以强烈结合细胞质区域，在肿瘤诊断、长期细胞追踪、药物代谢和药物递送系统中可能具有广阔的应用前景。
