Zhai Yi, Dasog Mita, Snitynsky Ryan B, Purkait Tapas K, Aghajamali Maryam, Hahn Allison H, Sturdy Christopher B, Lowary Todd L, Veinot Jonathan G C
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
J Mater Chem B. 2014 Dec 21;2(47):8427-8433. doi: 10.1039/c4tb01161a. Epub 2014 Oct 29.
Herein, we report the straightforward synthesis, photoluminescent properties, and cell imaging studies of d-mannose and l-alanine functionalized silicon nanocrystals (SiNCs). Tailoring nanocrystal surface functionalization is essential to interfacing SiNCs with their environment and rendering them stable - surface modification also offers the opportunity to target specific cell types for imaging. A simple and versatile surface modification procedure was developed to tether biomolecules onto the SiNC surfaces and render them water-soluble. The presented approach is precious metal-catalyst free, straightforward, and provides carbohydrate and amino acid functionalized SiNCs. The functionalized SiNCs have been investigated by fluorescence microscopy and our results indicate that they can be internalized by MCF-7 human breast cancer cells as shown in the cell imaging studies. The obtained SiNCs were characterized using FTIR, XPS, PL, and TEM.
在此，我们报告了d-甘露糖和l-丙氨酸功能化硅纳米晶体（SiNCs）的直接合成、光致发光特性及细胞成像研究。定制纳米晶体表面功能化对于使SiNCs与其环境相互作用并使其稳定至关重要——表面修饰还为靶向特定细胞类型进行成像提供了机会。我们开发了一种简单通用的表面修饰程序，将生物分子连接到SiNC表面并使其具有水溶性。所提出的方法无需贵金属催化剂，操作简便，可提供碳水化合物和氨基酸功能化的SiNCs。通过荧光显微镜对功能化的SiNCs进行了研究，我们的结果表明，如细胞成像研究所显示的，它们可被MCF-7人乳腺癌细胞内化。使用傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）、光致发光（PL）和透射电子显微镜（TEM）对所获得的SiNCs进行了表征。
