O'Neil Crystal L, Stine Keith J, Demchenko Alexei V
Department of Chemistry and Biochemistry, University of Missouri - St. Louis, One University Boulevard, St. Louis, Missouri, USA.
J Carbohydr Chem. 2018;37(4):225-249. doi: 10.1080/07328303.2018.1462372. Epub 2018 Apr 27.
Carbohydrates are an important class of biomolecules which are involved in a multitude of cellular functions. In the field of glycomics, the structure and function of various carbohydrates, oligosaccharides, glycans and their conjugates are constantly under investigation. In the continuing quest to understand the roles of carbohydrates in their interactions with proteins, immunogens, and other cell-surface carbohydrates, scientists have developed methods for observing the effects of specific saccharide sequences on various cellular components. Carbohydrate immobilization has allowed researchers to study the impact of specific sequences, leading to a deeper understanding of many cellular processes. The goal of this review is to highlight the chemical reactions and interactions that have been used for glycan immobilization.
碳水化合物是一类重要的生物分子，参与多种细胞功能。在糖组学领域，各种碳水化合物、寡糖、聚糖及其缀合物的结构和功能一直是研究的重点。在不断探索碳水化合物与蛋白质、免疫原及其他细胞表面碳水化合物相互作用的过程中，科学家们开发出了观察特定糖序列对各种细胞成分影响的方法。碳水化合物固定化使研究人员能够研究特定序列的影响，从而更深入地了解许多细胞过程。本综述的目的是突出用于聚糖固定化的化学反应和相互作用。
