Zhai Qingfeng, Zhang Siqi, Jiang Hong, Wei Qin, Wang Erkang, Wang Jiahai
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
J Mater Chem B. 2014 Oct 7;2(37):6371-6377. doi: 10.1039/c4tb00844h. Epub 2014 Aug 14.
In this article, we propose a new method for selective detection of Hg based on a biomimetic nanopore sensing platform in combination with single-walled carbon nanotubes (SWNTs). As is well known, folded DNA in the presence of Hg can be separated from single-stranded DNA through SWNTs, and the folded DNA can be quantitated with cone-shaped nanopore whose surface was coated by polyethyleneimine (PEI)/Zr. Both sensitivity and selectivity based on this paradigm can be guaranteed without immobilization of probes on the nanopore surface. This approach can warrant the detection limit for Hg down to 8.3 nM (S/N = 3) with high selectivity against other metal ions. Moreover, the application of the sensor for lake water shows that the proposed method works well for real samples. This research demonstrates an alternative approach to detect targets of interest that holds high prospects for detecting other biomolecules or metal ions in the near future.
在本文中，我们提出了一种基于仿生纳米孔传感平台与单壁碳纳米管（SWNTs）相结合的汞选择性检测新方法。众所周知，在汞存在的情况下，折叠的DNA可以通过单壁碳纳米管与单链DNA分离，并且可以用表面涂有聚乙烯亚胺（PEI）/锆的锥形纳米孔对折叠的DNA进行定量。基于这种模式的灵敏度和选择性都可以得到保证，而无需在纳米孔表面固定探针。这种方法可以保证汞的检测限低至8.3 nM（信噪比 = 3），对其他金属离子具有高选择性。此外，该传感器在湖水中的应用表明，所提出的方法对实际样品效果良好。这项研究展示了一种检测目标物的替代方法，在不久的将来检测其他生物分子或金属离子方面具有很高的前景。
