State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
Biosens Bioelectron. 2011 Aug 15;26(12):4656-60. doi: 10.1016/j.bios.2011.03.026. Epub 2011 Apr 2.
In this article, carbon nanoparticles (CNPs) were used as a novel fluorescent sensing platform for highly sensitive and selective Hg(2+) detection. To the best of our knowledge, this is the first example of CNPs obtained from candle soot used in this type of sensor. The general concept used in this approach is based on that adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by CNP via π-π stacking interactions between DNA bases and CNP leads to substantial dye fluorescence quenching; however, in the presence of Hg(2+), T-Hg(2+)-T induced hairpin structure does not adsorb on CNP and thus retains the dye fluorescence. A detection limit as low as 10nM was achieved. The present CNP-based biosensor for Hg(2+) detection exhibits remarkable specificity against other possible metal ions. Furthermore, superior selectivity performance was observed when Hg(2+) detection was carried out in the presence of a large amount of other interference ions. Finally, in order to evaluate its potential practical application, Hg(2+) detection was conducted with the use of lake water other than pure buffer and it is believed that it holds great promise for real sample analysis upon further development.
本文使用碳纳米粒子 (CNP) 作为一种新型荧光传感平台，用于高灵敏度和选择性的 Hg(2+) 检测。据我们所知，这是首次将蜡烛烟尘中得到的 CNP 应用于这种传感器中。该方法的基本原理是，通过 DNA 碱基与 CNP 之间的π-π 堆积相互作用，使荧光标记的单链 DNA（ssDNA）探针吸附在 CNP 上，导致染料荧光显著猝灭；然而，在存在 Hg(2+) 的情况下，T-Hg(2+)-T 诱导的发夹结构不会吸附在 CNP 上，从而保留了染料荧光。检测限低至 10nM。这种基于 CNP 的 Hg(2+) 检测生物传感器对其他可能的金属离子表现出显著的特异性。此外，在存在大量其他干扰离子的情况下进行 Hg(2+) 检测时，观察到优异的选择性性能。最后，为了评估其潜在的实际应用，在使用除纯缓冲液以外的湖水进行 Hg(2+) 检测，相信在进一步开发后，它在实际样品分析中具有很大的应用前景。
