Zhang Wei, Liu Jun, Shi Haigang, Liu Na, Yang Kun, Shi Lianxin, Gu Bin, Wang Huaiyu, Ji Junhui, Chu Paul K
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
J Mater Chem B. 2015 Mar 7;3(9):1856-1863. doi: 10.1039/c4tb02071e. Epub 2015 Jan 28.
Because of the complex plasma reactions and chemical structures of polymers, it is difficult to construct nitrogen functionalities controllably by plasma technology to attain the desirable biological outcome and hence, their effects on bone cells are sometimes ambiguous and even contradictory. In this study, argon plasma treatment is utilized to convert complex molecular chains into a pyrolytic carbon structure which possesses excellent cytocompatibility. The pyrolytic carbon then serves as a platform to prepare the desired nitrogen functionalities by nitrogen and hydrogen plasma immersion ion implantation. Primary, secondary, and tertiary amine groups can be produced selectively thus minimizing the chemical complexity and creation of multiple types of nitrogen functional groups that are often obtained by other fabrication methods. As a result of the excellent control of the nitrogen functionalities rendered by this plasma technique, the effects of individual nitrogen functionalities on the cytocompatibility and upregulation of bone marrow-derived mesenchymal stem cell (BMSC) osteogenesis can be investigated systematically. The tertiary amine functionalities exhibit the optimal efficiency pertaining to the modulation of the biological response, enhancement of osteogenesis related gene/protein expression, and calcification of the contacted BMSCs. Our results demonstrate that simple plasma technology can be conveniently employed to create the desirable nitrogen functionalities on orthopedic polymers to facilitate osseointegration and mitigate foreign body reactions.
由于聚合物复杂的等离子体反应和化学结构，利用等离子体技术可控地构建氮官能团以获得理想的生物学结果具有一定难度，因此，它们对骨细胞的影响有时并不明确甚至相互矛盾。在本研究中，采用氩等离子体处理将复杂的分子链转化为具有优异细胞相容性的热解碳结构。然后，热解碳作为一个平台，通过氮和氢等离子体浸没离子注入来制备所需的氮官能团。可以选择性地产生伯胺、仲胺和叔胺基团，从而最大限度地减少化学复杂性以及其他制造方法中常出现的多种类型氮官能团的产生。由于这种等离子体技术对氮官能团具有出色的控制能力，因此可以系统地研究单个氮官能团对骨髓间充质干细胞（BMSC）细胞相容性和骨生成上调的影响。叔胺官能团在调节生物学反应、增强骨生成相关基因/蛋白表达以及接触的BMSC钙化方面表现出最佳效率。我们的结果表明，简单的等离子体技术可方便地用于在骨科聚合物上创建所需的氮官能团，以促进骨整合并减轻异物反应。
