Hamilton J G, Comai K
Department of Pharmacology, Hoffmann-La Roche Inc., Nutley, NJ 07110.
Lipids. 1988 Dec;23(12):1146-9. doi: 10.1007/BF02535281.
A method is described for the separation of neutral lipid, free fatty acid and polar lipid classes using small (600 mg), prepacked silica Sep-Pak columns. Combinations of hexane and methyltertiarybutylether were used to progressively elute cholesteryl ester first then triglyceride from the column. After column acidification, fatty acids were eluted followed by cholesterol. Recoveries of these lipids were 96% or greater. Polar lipids were eluted from the column using combinations of methyltertiarybutylether, methanol and ammonium acetate. Phospholipid classes could not be separated completely from each other. Phosphatidylethanolamine and phosphatidylinositol eluted together, whereas the more polar phosphatidylcholine, sphingomyelin and lysophosphatidylcholine were eluted as a second fraction. Recoveries of each phospholipid was greater than 98%.
描述了一种使用小型（600毫克）预填充硅胶Sep - Pak柱分离中性脂质、游离脂肪酸和极性脂质类别的方法。己烷和甲基叔丁基醚的组合用于先逐步从柱上洗脱胆固醇酯，然后洗脱甘油三酯。柱酸化后，洗脱脂肪酸，接着洗脱胆固醇。这些脂质的回收率为96%或更高。使用甲基叔丁基醚、甲醇和醋酸铵的组合从柱上洗脱极性脂质。磷脂类彼此不能完全分离。磷脂酰乙醇胺和磷脂酰肌醇一起洗脱，而极性更强的磷脂酰胆碱、鞘磷脂和溶血磷脂酰胆碱作为第二部分洗脱。每种磷脂的回收率大于98%。
