Li Xian, Xue Wenjiao, Liu Yannan, Fan Daidi, Zhu Chenhui, Ma Xiaoxuan
Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
J Mater Chem B. 2015 Jun 21;3(23):4742-4755. doi: 10.1039/c5tb00408j. Epub 2015 May 26.
In this study, we designed multifunctionalized hydrogel scaffolds and injectable particles based on high-molecular-weight (M) pullulan and human-like collagen (HLC) crosslinked with 1,4-butanediol diglycidyl ether (BDDE) for combination therapy tissue restoration. The properties of the pullulan/BDDE (PB) and pullulan/BDDE/human-like collagen (PBH) hydrogels were characterized via swelling ratio measurements, mechanical tests, and enzymatic degradation in vitro and via subcutaneous injections in vivo. The results demonstrate that the dry hydrogels completely returned to their original state in deionized water. The elastic modulus of the PBH53 dry hydrogels is higher than that of the other hydrogels after exposure to bending stress and compression stress with a maximum value of 7858.93 MPa. In addition, the in vitro live/dead staining and cell adhesion of the PBH hydrogels exhibited a superior fibroblast morphology without high levels of cell death, which were considerably better than those of PB hydrogels. In vivo, PB and PBH particles with good biocompatibility and anti-biodegradation were successfully prepared via the granulation of wet PB and PBH hydrogels for efficient subcutaneous injection in Kunming mice and New Zealand rabbits. Therefore, the PB and PBH hydrogels were found to be acceptable, safe, soft materials for use in skin restoration, cartilage treatment, and lacrimal dryness therapy.
在本研究中，我们基于高分子量（M）普鲁兰多糖和人源化胶原蛋白（HLC），设计了多功能水凝胶支架和可注射颗粒，它们通过与1,4 - 丁二醇二缩水甘油醚（BDDE）交联用于联合治疗组织修复。通过溶胀率测量、力学测试、体外酶降解以及体内皮下注射来表征普鲁兰多糖/BDDE（PB）和普鲁兰多糖/BDDE/人源化胶原蛋白（PBH）水凝胶的性能。结果表明，干水凝胶在去离子水中能完全恢复到其原始状态。在承受弯曲应力和压缩应力后，PBH53干水凝胶的弹性模量高于其他水凝胶，最大值为7858.93 MPa。此外，PBH水凝胶的体外活/死染色和细胞黏附显示出良好的成纤维细胞形态，且细胞死亡水平不高，明显优于PB水凝胶。在体内，通过对湿PB和PBH水凝胶进行造粒，成功制备了具有良好生物相容性和抗生物降解性的PB和PBH颗粒，用于在昆明小鼠和新西兰兔体内进行高效皮下注射。因此，发现PB和PBH水凝胶是用于皮肤修复、软骨治疗和泪液干燥治疗的可接受、安全且柔软的材料。
