Zhang Jingjing, Ma Xiaoxuan, Fan Daidi, Zhu Chenhui, Deng Jianjun, Hui Junfeng, Ma Pei
Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
Mater Sci Eng C Mater Biol Appl. 2014 Oct;43:547-54. doi: 10.1016/j.msec.2014.07.058. Epub 2014 Jul 27.
Injectable hydrogel plays an important role in soft tissue filling and repair. We report an injectable hydrogel based on hyaluronic acid (HA) and human-like collagen (HLC), both with favorable biocompatibility and biodegradability. These two types of biomacromolecules were crosslinked with 1,4-butanediol diglycidyl ether to form a three-dimensional network. The redundant crosslinker was removed by dialysis and distillation. An HA-based hydrogel prepared by the same method was used as a control. The cytocompatibility was studied with a Cell Counting Kit-8 (CCK-8) test. Carbazole colorimetry was used to analyze the in vitro degradation rate. The histocompatibility was evaluated by hematoxylin and eosin (H&E) staining analysis and immunohistochemical analysis. The CCK-8 assay demonstrated that the HA/HLC hydrogel was less cytotoxic than the HA-based hydrogel and could promote baby hamster kidney cell (BHK) proliferation. The cell adhesion indicated that BHK could grow well on the surface of the materials and maintain good cell viability. The in vitro degradation test showed that the HA/HLC hydrogel had a longer degradation time and an excellent antienzyme ability. In vivo injection showed that there was little inflammatory response to HA/HLC after 1, 2, and 4 weeks. Therefore, the HA/HLC hydrogel is a promising biomaterial for soft tissue filling and repair.
可注射水凝胶在软组织填充和修复中发挥着重要作用。我们报道了一种基于透明质酸（HA）和类人胶原蛋白（HLC）的可注射水凝胶，二者均具有良好的生物相容性和生物降解性。这两种生物大分子通过1,4-丁二醇二缩水甘油醚交联形成三维网络。多余的交联剂通过透析和蒸馏去除。用相同方法制备的基于HA的水凝胶用作对照。通过细胞计数试剂盒-8（CCK-8）试验研究细胞相容性。采用咔唑比色法分析体外降解率。通过苏木精和伊红（H&E）染色分析及免疫组织化学分析评估组织相容性。CCK-8试验表明，HA/HLC水凝胶的细胞毒性低于基于HA的水凝胶，并且能够促进幼仓鼠肾细胞（BHK）增殖。细胞黏附表明BHK能够在材料表面良好生长并保持良好的细胞活力。体外降解试验表明，HA/HLC水凝胶具有更长的降解时间和出色的抗酶能力。体内注射显示，在1、2和4周后，对HA/HLC几乎没有炎症反应。因此，HA/HLC水凝胶是一种用于软组织填充和修复的有前景的生物材料。
