Synthesis, characterization and biological evaluation of a new photoactive hydrogel against Gram-positive and Gram-negative bacteria.

In 2013, the World Health Organization reported that 884 million people lack access to clean potable water. Photodynamic antimicrobial chemotherapy (PACT) is a very promising alternative to conventional antibiotics for the efficient inactivation of pathogenic microorganisms. We report the synthesis, characterization and antibacterial activity of a polyacrylamide-based hydrogel (7), with a new photoactive phenothiazinium compound (6) immobilized on it, to be used as a novel water-sterilizing device. The hydrogel was characterized by IR and scanning electron microscopy and incorporation of the dye confirmed by UV-visible spectroscopy. Antibacterial tests using the recombinant bioluminescent Gram-positive Staphylococcus aureus RN4220 and Gram-negative Escherichia coli DH5α were performed to assess the ability of the hydrogel to inactivate bacterial strains in solution. The hydrogel is characterized by a non-ordered microporous structure and is able to generate reactive oxygen species. The hydrogel is able to inactivate planktonic cells of the S. aureus and E. coli (3.3 log and 2.3 log killing, respectively) after 25 min of irradiation with white light at 14.5 mW cm. The contact surface does not influence the kill rates while the killing rate increased by increasing the total amount of the hydrogel (0.27 log drop to 1.65 log drop with 0.5 mg cm to 2.5 mg cm of total amount of dye). The hydrogel was found to be active for four cycles, suggesting the possibility of reuse and it was shown to be active against both Gram-positive and Gram-negative species with no leaching of the active molecule.