Zhao Xia, Shen Yi, Adogla Enoch A, Viswanath Anand, Tan Rui, Benicewicz Brian C, Greytak Andrew B, Lin Yuan, Wang Qian
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
J Mater Chem B. 2016 Apr 14;4(14):2421-2427. doi: 10.1039/c6tb00263c. Epub 2016 Mar 17.
We report a general method for the preparation of quantum dot-labeled viruses through a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. The quantum dot sample was functionalized with methacrylate-based polymeric imidazole ligands (MA-PILs) bearing dibenzocyclooctyne groups. Enveloped measles virus was labeled with azide groups through the metabolic incorporation of a choline analogue into the host cell membrane, and then linked with the modified QDs. The virus retained its infectious ability against host cells after the modification with MA-PIL capped QDs.
我们报道了一种通过应变促进的叠氮化物-炔烃环加成（SPAAC）反应制备量子点标记病毒的通用方法。量子点样品用带有二苯并环辛炔基团的基于甲基丙烯酸酯的聚合物咪唑配体（MA-PILs）进行功能化。通过将胆碱类似物代谢掺入宿主细胞膜，使包膜麻疹病毒带有叠氮基团，然后与修饰的量子点连接。用MA-PIL封端的量子点修饰后，病毒保留了对宿主细胞的感染能力。
