Yang Yuling, Zhu Genxing, Wang Guangchuan, Li Yali, Tang Ruikang
Center for Biomaterials and Biopathways, Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang 310027, China.
J Mater Chem B. 2016 Jul 21;4(27):4726-4731. doi: 10.1039/c6tb01355d. Epub 2016 Jun 24.
Biomolecules, especially enzymes, usually have poor thermal and operational stability as well as limited reuse cycles, which greatly limit their industrial practices. Inspired by the biomineralization strategy evolved by natural organisms, we suggest nanohybrid enzyme formulation by in situ encapsulating enzyme loaded functional FeO@C nanoparticles with silica. By using glucose oxidase (GOD) as an example, we demonstrate that the obtained enzyme-material hybrids are featured by their significantly enhanced operational and thermal stabilities, which exhibit a relatively steady catalytic ability in a board range of 25 °C to 65 °C. Even after 4 h of incubation at 55 °C, the GOD-material composites still retain 77% of their initial activity while the native ones only retain 30%. Besides, the nanohybrids show excellent reusability because the magnetic character of the integrated FeO particles facilitates the enzyme separation and recycle. This attempt provides a valuable approach for biological improvement by using functional materials.
生物分子，尤其是酶，通常具有较差的热稳定性和操作稳定性，以及有限的重复使用周期，这极大地限制了它们的工业应用。受天然生物体进化出的生物矿化策略的启发，我们提出通过用二氧化硅原位封装负载酶的功能性FeO@C纳米颗粒来制备纳米复合酶制剂。以葡萄糖氧化酶（GOD）为例，我们证明所获得的酶-材料杂化物具有显著增强的操作稳定性和热稳定性，在25℃至65℃的宽温度范围内表现出相对稳定的催化能力。即使在55℃孵育4小时后，GOD-材料复合物仍保留其初始活性的77%，而天然酶仅保留30%。此外，纳米杂化物显示出优异的可重复使用性，因为整合的FeO颗粒的磁性有助于酶的分离和循环利用。这种尝试为利用功能材料进行生物改良提供了一种有价值的方法。
