Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education, Wuhan University of Technology, 430070, Wuhan, China.
Biotechnol Lett. 2010 Jun;32(6):817-21. doi: 10.1007/s10529-010-0217-9. Epub 2010 Feb 14.
Glucose oxidase (GOD) was covalently immobilized onto Fe(3)O(4)/SiO(2) magnetic nanoparticles (FSMNs) using glutaraldehyde (GA). Optimal immobilization was at pH 6 with 3-aminopropyltriethoxysilane at 2% (v/v), GA at 3% (v/v) and 0.143 g GOD per g carrier. The activity of immobilized GOD was 4,570 U/g at pH 7 and 50 degrees C. The immobilized GOD retained 80% of its initial activity after 6 h at 45 degrees C while free enzyme retained only 20% activity. The immobilized GOD maintained 60% of its initial activity after 6 cycles of repeated use and retained 75% of its initial activity after 1 month at 4 degrees C whereas free enzymes retained 62% of its activity.
葡萄糖氧化酶（GOD）通过戊二醛（GA）共价固定在 Fe(3)O(4)/SiO(2)磁性纳米粒子（FSMNs）上。最佳固定条件为 pH 6，3-氨丙基三乙氧基硅烷（APTES）体积分数 2%（v/v），GA 体积分数 3%（v/v），每克载体固定 0.143 克 GOD。固定化 GOD 在 pH 7 和 50°C 时的酶活为 4,570 U/g。固定化 GOD 在 45°C 下保持 80%的初始酶活 6 小时，而游离酶仅保留 20%的活性。固定化 GOD 在重复使用 6 次后保持 60%的初始酶活，在 4°C 下放置 1 个月后保持 75%的初始酶活，而游离酶保留 62%的活性。
