Wang Yang, Hou Chen, Zhang Yun, He Fu, Liu Mingzhu, Li Xiaoli
State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, College of Resources and Environment, Institute of Biochemical Engineering & Environmental Technology, Gansu Key Laboratory for Environmental Pollution Prediction and Control, College of Earth and Environmental Sciences, Lanzhou University, Lanzhou 730000, China.
J Mater Chem B. 2016 Jun 7;4(21):3695-3702. doi: 10.1039/c6tb00276e. Epub 2016 May 3.
A novel amperometric biosensor for glucose detection was fabricated based on a mimetic multi-enzyme system by combing mimetic enzymes (metal-organic frameworks, MOFs) and glucose oxidase (GOx). The microcapsule constructed with polydopamine (PDA) and ZIF-8 using CaCO templates could be utilized not only to provide the biocompatible and non-toxic microenvironment to immobilize GOx, but also as an efficient mimetic enzyme because of the peroxidase-like properties of ZIF-8. The graphene nano-sheets (rGO) were bonded to the PDA/ZIF-8 microcapsules to accelerate the electron transfer between the microcapsule and the electrode, and the resulted PDA/ZIF-8@rGO showed excellent electro-catalytic activity towards HO. The mimetic multi-enzyme system was fabricated when GOx was immobilized in the as-prepared microcapsule, and the developed GOx/PDA/ZIF-8@rGO/GCE biosensor exhibited extraordinary electro-detection performance for glucose with two wide linear ranges from 1 μM to 1.2 mM and 1.2 mM to 3.6 mM, and the limit of detection was 0.333 μM (S/N = 3). Moreover, the good selectivity for glucose and satisfactory detection results in real samples of the prepared biosensor were also demonstrated.
基于模拟多酶系统，通过将模拟酶（金属有机框架，MOFs）与葡萄糖氧化酶（GOx）相结合，制备了一种用于葡萄糖检测的新型安培生物传感器。以碳酸钙（CaCO）为模板，用聚多巴胺（PDA）和ZIF-8构建的微胶囊不仅可用于提供生物相容性和无毒的微环境来固定GOx，而且由于ZIF-8具有类过氧化物酶性质，还可作为一种高效的模拟酶。将石墨烯纳米片（rGO）与PDA/ZIF-8微胶囊结合，以加速微胶囊与电极之间的电子转移，所得的PDA/ZIF-8@rGO对H₂O₂表现出优异的电催化活性。当将GOx固定在制备好的微胶囊中时，构建了模拟多酶系统，所制备的GOx/PDA/ZIF-8@rGO/玻碳电极（GCE）生物传感器对葡萄糖具有出色的电检测性能，具有1 μM至1.2 mM和1.2 mM至3.6 mM两个宽线性范围，检测限为0.333 μM（S/N = 3）。此外，还证明了所制备的生物传感器对葡萄糖具有良好的选择性以及在实际样品中具有令人满意的检测结果。
