Li Wenhua, Cai Yurong, Zhong Qiwei, Yang Ying, Kundu Subhas C, Yao Juming
The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
J Mater Chem B. 2016 Jan 14;4(2):340-347. doi: 10.1039/c5tb02328a. Epub 2015 Dec 15.
Silk protein sericin based organic-inorganic hybrid microcapsules are fabricated by incubating sericin microcapsules with a supersaturated calcium phosphate solution containing citric acid. A mineral hydroxyapatite shell is formed on the surface of the microcapsules. The thickness of the mineralized shell is dependant on the mineralization time. The amylum as a model cargo is encapsulated into the microcapsules with/without the presence of a mineralized shell. The release behavior of amylum from the microcapsules is investigated under different external environments. The results show that the release speed of amylum from the hybrid microcapsules is slower than from the sericin microcapsules under different pH and ionic strength conditions. This indicates that the stability of the hybrid microcapsules is improved due to the presence of the hydroxyapatite shell. The mineral shell provides a good protection to the microcapsule structure and carries goods against external harsh environment. In addition, cell cultures show a good cytocompatibility of the hybrid microcapsules. The natural microcapsules having an inorganic mineral shell cover may potentially act as drug delivery and encapsulating bioactive molecule systems.
基于丝蛋白丝胶的有机-无机杂化微胶囊是通过将丝胶微胶囊与含有柠檬酸的过饱和磷酸钙溶液孵育制备而成。在微胶囊表面形成了一层矿物羟基磷灰石壳。矿化壳的厚度取决于矿化时间。以淀粉作为模型货物，在有/无矿化壳的情况下将其封装到微胶囊中。研究了在不同外部环境下淀粉从微胶囊中的释放行为。结果表明，在不同的pH和离子强度条件下，淀粉从杂化微胶囊中的释放速度比从丝胶微胶囊中的释放速度慢。这表明由于羟基磷灰石壳的存在，杂化微胶囊的稳定性得到了提高。矿物壳为微胶囊结构提供了良好的保护，并能携带货物抵御外部恶劣环境。此外，细胞培养显示杂化微胶囊具有良好的细胞相容性。具有无机矿物壳覆盖的天然微胶囊可能潜在地用作药物递送和封装生物活性分子系统。
