Ding Sheng-Gang, Yu Lei, Wang Long-Hai, Wang Lin-Ding, Yu Zhi-Qiang, You Ye-Zi
Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
J Mater Chem B. 2016 Oct 21;4(39):6462-6467. doi: 10.1039/c6tb01891b. Epub 2016 Sep 26.
Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery. However, low transfection efficiency and inevitable toxicity are challenges of cationic polymers. In this study, we prepared a novel Janus dendritic polymer via a one-step Michael addition reaction of branched low-molecular-weight polyethylenimine (PEI, 1800 Da) and dendritic polythioether. The amphiphilic dendritic polymer can self-assemble into stable nanomicelles with high surface charge potential (+91.8 mV). The obtained nanomicelles can be used as a gene delivery vector, which exhibits a higher gene transfection efficiency and much lower cytotoxicity as compared with gold standard PEI (branched, 25 kDa).
聚阳离子具有高DNA凝聚能力、低免疫原性和良好的适应性，这使其在基因递送方面具有广阔前景。然而，低转染效率和不可避免的毒性是阳离子聚合物面临的挑战。在本研究中，我们通过支化低分子量聚乙烯亚胺（PEI，1800 Da）与树枝状聚硫醚的一步迈克尔加成反应制备了一种新型的Janus树枝状聚合物。这种两亲性树枝状聚合物能自组装成具有高表面电荷电位（+91.8 mV）的稳定纳米胶束。所制备的纳米胶束可用作基因递送载体，与金标准PEI（支化，25 kDa）相比，其表现出更高的基因转染效率和更低的细胞毒性。
