Lu Lihua, Wang Wanhe, Yang Chao, Kang Tian-Shu, Leung Chung-Hang, Ma Dik-Lung
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China.
J Mater Chem B. 2016 Nov 14;4(42):6791-6796. doi: 10.1039/c6tb02316a. Epub 2016 Oct 17.
A systematic study was performed to investigate the relationship between molecular structure and G-quadruplex sensing ability for a series of structurally related cyclometallated iridium(iii) complexes. The novel complex 7 was used to construct a label-free luminescent switch-on G-quadruplex-based assay for RNase H activity in buffered solution. The constructed detection platform was simple, low-cost and highly sensitive for RNase H, and functioned effectively in the presence of cellular debris.
进行了一项系统研究，以考察一系列结构相关的环金属化铱（III）配合物的分子结构与G-四链体传感能力之间的关系。新型配合物7被用于构建一种基于G-四链体的无标记荧光开启检测法，用于缓冲溶液中RNase H活性的检测。所构建的检测平台对RNase H而言简单、低成本且高度灵敏，并且在存在细胞碎片的情况下能有效发挥作用。
