基于 G-四链体选择性铱（III）配合物的无标记荧光开启型探针用于检测赭曲霉毒素 A。
Label-Free Luminescent Switch-On Probe for Ochratoxin A Detection Using a G-Quadruplex-Selective Iridium(III) Complex.
机构信息
†Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
‡State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
出版信息
ACS Appl Mater Interfaces. 2015 Apr 22;7(15):8313-8. doi: 10.1021/acsami.5b01702. Epub 2015 Apr 9.
A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2=0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.
合成了一个包含六种发光 Ir(III) 配合物的库，并研究了它们作为 G-四链体 DNA 探针的能力。发现新型 Ir(III) 配合物 1 对 G-四链体结构具有选择性，并随后用于在水溶液中构建无标记 G-四链体基于赭曲霉毒素 A (OTA) 传感平台。该测定法在 0 到 60 nM 的范围内对 OTA 呈线性（R2=0.9933），OTA 的检测限为 5 nM。此外，该测定法对 OTA 具有高度选择性，超过了其结构相关的类似物。
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