Ozturk Kirbay Fatma, Ayranci Rukiye, Ak Metin, Odaci Demirkol Dilek, Timur Suna
Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkey.
J Mater Chem B. 2017 Sep 14;5(34):7118-7125. doi: 10.1039/c7tb01716b. Epub 2017 Aug 17.
We report here the electrochemical co-polymerization of two functional monomers, one containing fluorescent rhodamine dye (RF) and the other monomer having amine groups (RD), onto electroactive Indium Tin Oxide (ITO) glass. After one step preparation of these surfaces, a three peptide called ArginylGlysylAspartic acid (RGD) was immobilized via EDC chemistry by using amine groups (P(RF-co-RD)/RGD) of the co-polymer, for further use in various bio-applications such as cell adhesion and imaging as well as electrochemical cell sensing. The resultant RGD bound and also fluorescent platforms were utilized as targeted adhesion materials towards integrin avb3 receptor positive (U87-MG) cells and the selectivity was checked by using HaCaT cells as a control. Finally, electrochemical measurements were carried out to characterize step by step surface modification and detection of cell attachment. As a result, P(RF-co-RD)/RGD is a promising material for multi-purpose uses, such as fluorescence imaging without the need for an additional dye for cell visualization and as a targeted adhesion and electrochemical cell sensing platform.
我们在此报告两种功能单体在具有电活性的氧化铟锡（ITO）玻璃上的电化学共聚情况，其中一种单体含有荧光罗丹明染料（RF），另一种单体带有胺基（RD）。在一步制备这些表面之后，通过使用共聚物的胺基（P(RF-co-RD)/RGD），经由EDC化学法固定了一种名为精氨酰甘氨酰天冬氨酸（RGD）的三肽，以进一步用于各种生物应用，如细胞黏附、成像以及电化学细胞传感。所得的RGD结合且具有荧光的平台被用作针对整合素αvβ3受体阳性（U87-MG）细胞的靶向黏附材料，并以HaCaT细胞作为对照来检查其选择性。最后，进行电化学测量以逐步表征表面修饰和细胞附着的检测。结果表明，P(RF-co-RD)/RGD是一种有前途的多功能材料，例如无需额外染料即可用于细胞可视化的荧光成像，以及作为靶向黏附和电化学细胞传感平台。
