Li Yan, Luo Zuyuan, Xu Xiao, Li Yongliang, Zhang Siqi, Zhou Ping, Sui Yi, Wu Minjie, Luo En, Wei Shicheng
Central Laboratory, School and Hospital of Stomatology, Peking University, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, China.
J Mater Chem B. 2017 Sep 14;5(34):7153-7163. doi: 10.1039/c7tb01732d. Epub 2017 Aug 18.
Several bone diseases, including arthritis, fracture and osteoporosis, have a pathophysiologically important inflammatory component. Sustained inflammation can result in delayed bone healing. Therefore, to promote bone repair, it is important to inhibit inflammatory bone erosion and suppress pro-inflammatory mediators. In this study, aspirin significantly enhanced immunomodulation and osteogenic differentiation in human mesenchymal stem cells (hMSCs). Additionally, an osteogenic BFP-1 peptide-decorated substrate (PS-PEP) enhanced osteogenic differentiation of aspirin-treated hMSCs compared to a pristine substrate. Alkaline phosphatase assay, quantitative real-time polymerase chain reaction, immunostaining and Alizarin Red S staining revealed that aspirin-treated hMSCs cultured on PS-PEP exhibited enhanced osteogenesis compared with untreated cells. Thus, we report here that the anti-inflammatory and osteogenic effects of aspirin promote the activity and osteogenesis of hMSCs. The combination of aspirin and an osteogenic BFP-1 peptide-decorated substrate suppresses the production of pro-inflammatory mediators and promotes osteogenic differentiation of hMSCs; therefore, this novel strategy has potential for application in cell therapy and bone tissue engineering.
包括关节炎、骨折和骨质疏松症在内的几种骨疾病，在病理生理学上具有重要的炎症成分。持续的炎症会导致骨愈合延迟。因此，为促进骨修复，抑制炎症性骨侵蚀和抑制促炎介质很重要。在本研究中，阿司匹林显著增强了人间充质干细胞（hMSCs）的免疫调节和成骨分化。此外，与原始基质相比，一种成骨BFP-1肽修饰的基质（PS-PEP）增强了经阿司匹林处理的hMSCs的成骨分化。碱性磷酸酶测定、定量实时聚合酶链反应、免疫染色和茜素红S染色显示，在PS-PEP上培养的经阿司匹林处理的hMSCs与未处理的细胞相比，成骨增强。因此，我们在此报告，阿司匹林的抗炎和成骨作用促进了hMSCs的活性和成骨。阿司匹林与成骨BFP-1肽修饰基质的组合抑制了促炎介质的产生，并促进了hMSCs的成骨分化；因此，这种新策略在细胞治疗和骨组织工程中具有应用潜力。
