Di Yuntao, Wang Cunyang, Zhu Huixue, Yu Suxiang, Ren Yixing, Li Xiaoming
Department of Neurosurgery, the Fourth Central Hospital of Baoding City, Baoding Hebei, 072350, P.R.China.
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Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2021 Mar 15;35(3):286-294. doi: 10.7507/1002-1892.202009014.
To observe and compare the effects of peptides on the repair of rabbit skull defects through two different binding modes of non-covalent and covalent, and the combination of carboxyl (-COOH) and amino (-NH ) groups with materials.
Twenty-one 3-month-old male ordinary New Zealand white rabbits were numbered 1 to 42 on the left and right parietal bones. They were divided into 5 groups using a random number table, the control group (group A, 6 sides) and the material group 1, 2, 3, 4 (respectively group B, C, D, E, 9 sides in each group). All animals were prepared with 12-mm-diameter skull defect models, and bone morphogenetic protein 2 (BMP-2) non-covalently bound multiwalled carbon nanotubes (MWCNT)-COOH+poly ( -lactide) (PLLA), BMP-2 non-covalently bound MWCNT-NH +PLLA, BMP-2 covalently bound MWCNT-COOH+PLLA, and BMP-2 covalently bound MWCNT-NH +PLLA were implanted into the defects of groups B, C, D, and E, respectively. At 4, 8, and 12 weeks after operation, the samples were taken for CT scanning and three-dimensional reconstruction, the ratio of bone tissue regeneration volume to total volume and bone mineral density were measured, and the histological observation of HE staining and Masson trichrome staining were performed to quantitatively analyze the volume ratio of new bone tissue.
CT scanning and three-dimensional reconstruction showed that with the extension of time, the defects in groups A-E were filled gradually, and the defect in group E was completely filled at 12 weeks after operation. HE staining and Masson trichrome staining showed that the volume of new bone tissue in each group gradually increased with time, and regenerated mature bone tissue appeared in groups D and E at 12 weeks after operation. Quantitative analysis showed that at 4, 8, and 12 weeks after operation, the ratio of bone tissue regeneration volume to total volume, bone mineral density, and the volume ratio of new bone tissue increased gradually over time; and at each time point, the above indexes increased gradually from group A to group E, and the differences between groups were significant ( <0.05).
Through covalent binding and using -NH to bound peptides with materials, the best bone repair effect can be achieved.
通过非共价和共价两种不同结合模式以及羧基（-COOH）和氨基（-NH₂）与材料的结合，观察并比较肽对兔颅骨缺损修复的影响。
选取21只3月龄雄性普通新西兰白兔，左右顶骨分别编号为1至42。采用随机数字表法将其分为5组，即对照组（A组，6侧）和材料组1、2、3、4（分别为B组、C组、D组、E组，每组9侧）。所有动物均制备直径为12 mm的颅骨缺损模型，将骨形态发生蛋白2（BMP-2）非共价结合的多壁碳纳米管（MWCNT）-COOH+聚左旋乳酸（PLLA）、BMP-2非共价结合的MWCNT-NH₂+PLLA、BMP-2共价结合的MWCNT-COOH+PLLA以及BMP-2共价结合的MWCNT-NH₂+PLLA分别植入B、C、D、E组的缺损处。术后4、8、12周取材进行CT扫描及三维重建，测量骨组织再生体积与总体积的比值及骨密度，并进行HE染色和Masson三色染色的组织学观察，定量分析新生骨组织的体积比。
CT扫描及三维重建显示，随着时间延长，A-E组缺损逐渐被填充，E组缺损在术后12周完全被填充。HE染色和Masson三色染色显示，各组新生骨组织体积随时间逐渐增加，D组和E组在术后12周出现再生成熟骨组织。定量分析显示，术后4、8、12周，骨组织再生体积与总体积的比值、骨密度及新生骨组织体积比随时间逐渐增加；且在各时间点，上述指标从A组到E组逐渐升高，组间差异有统计学意义（P<0.05）。
通过共价结合并利用-NH₂将肽与材料结合，可获得最佳的骨修复效果。
