Kulanthaivel Senthilguru, Rathnam V S Sharan, Agarwal Tarun, Pradhan Susanta, Pal Kunal, Giri Supratim, Maiti Tapas K, Banerjee Indranil
Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
J Mater Chem B. 2017 Jun 14;5(22):4177-4189. doi: 10.1039/c7tb00390k. Epub 2017 May 17.
There is a dearth of biologically active matrices for the encapsulation of bone cells. Here, we hypothesize that the use of gum tragacanth (GT) with alginate might improve the biological properties of calcium alginate (CA) beads, a common cell encapsulation system. We show that the incorporation of GT in the bead-composition significantly improves the molecular transport, swelling and degradation properties of the CA bead. Although no significant molecular interaction between GT and CA was found, a decrease in the concentration of calcium with an increase in GT concentration was noticed. We show that the presence of GT in the bead-composition resulted in improved viability, proliferation, and differentiation of encapsulated bone cells. We further demonstrate that bone cell loaded CA-GT beads are capable of inducing angiogenesis. In conclusion, we prove that CA-GT beads are more osteo-conductive and proangiogenic in comparison to pure CA beads.
用于封装骨细胞的生物活性基质匮乏。在此，我们假设将刺梧桐树胶（GT）与海藻酸盐一起使用可能会改善海藻酸钙（CA）微珠（一种常见的细胞封装系统）的生物学特性。我们表明，在微珠成分中加入GT可显著改善CA微珠的分子转运、溶胀和降解特性。尽管未发现GT与CA之间存在显著的分子相互作用，但随着GT浓度的增加，钙浓度有所降低。我们表明，微珠成分中存在GT可提高封装骨细胞的活力、增殖和分化能力。我们进一步证明，负载骨细胞的CA-GT微珠能够诱导血管生成。总之，我们证明与纯CA微珠相比，CA-GT微珠具有更强的骨传导性和促血管生成能力。
