Komarala EswaraVara Prasadarao, Tyagi Himanshu, Thiyagarajan Shankar, Pradhan Lina, Aslam Mohammed, Bahadur Dhirendra
Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology Bombay, Mumbai, 400076, India.
J Mater Chem B. 2017 Jun 7;5(21):3852-3861. doi: 10.1039/c7tb00015d. Epub 2017 May 4.
Among inorganic nanomaterials, layered double hydroxides (LDHs) and gold nanoparticles (Au NPs) have received great attention in nanobiomedicine due to their unique properties. In this work, we have designed a nanohybrid of an LDH with Au NPs (LDH-Au) in order to use it for photothermal therapy, and optical and fluorescence imaging of cancer cells. The structural characteristics of the nanohybrid are investigated using X-ray diffraction, infrared spectroscopy, electron microscopy and elemental analyses. The extinction spectra of the nanohybrid exhibits broad absorption ranging from the visible to near infrared (NIR) region (500-1000 nm). The photothermal activity of the nanohybrid is explored using NIR laser irradiation. The electric field enhancement in the nanohybrid due to the interaction of Au NPs on the LDH is speculated through finite-difference time-domain (FDTD) calculations. The LDH-Au nanohybrid is found to be biocompatible with normal murine fibroblast (L929), human breast cancer (MCF-7) and cervical cancer (HeLa) cell lines up to a concentration of 1 mg mL. The nanohybrid is explored for in vitro photothermal therapy of MCF-7 and HeLa cell lines. As a photothermal agent, the nanohybrid shows that 10 min exposure to an 808 nm laser (500 mW) is adequate to inhibit about 70% of cancer cells. Further, the nanohybrid is tagged with FITC to study both optical and fluorescence imaging with MCF-7 cell lines. The results demonstrate that the LDH-Au nanohybrid provides an innovative approach to photothermal therapy, and optical and fluorescence imaging of cancer cells.
在无机纳米材料中，层状双氢氧化物（LDHs）和金纳米颗粒（Au NPs）因其独特的性质而在纳米生物医学领域备受关注。在这项工作中，我们设计了一种LDH与Au NPs的纳米杂化物（LDH-Au），以便将其用于光热治疗以及癌细胞的光学和荧光成像。使用X射线衍射、红外光谱、电子显微镜和元素分析对该纳米杂化物的结构特征进行了研究。该纳米杂化物的消光光谱在可见光到近红外（NIR）区域（500 - 1000 nm）呈现出宽吸收。利用近红外激光照射探索了该纳米杂化物的光热活性。通过有限时域差分（FDTD）计算推测了由于Au NPs在LDH上的相互作用而导致的纳米杂化物中的电场增强。发现LDH-Au纳米杂化物在浓度高达1 mg/mL时与正常小鼠成纤维细胞（L929）、人乳腺癌（MCF-7）和子宫颈癌（HeLa）细胞系具有生物相容性。对该纳米杂化物进行了MCF-7和HeLa细胞系的体外光热治疗研究。作为一种光热剂，该纳米杂化物表明，用808 nm激光（500 mW）照射10分钟足以抑制约70%的癌细胞。此外，该纳米杂化物用异硫氰酸荧光素（FITC）标记，以研究与MCF-7细胞系的光学和荧光成像。结果表明，LDH-Au纳米杂化物为光热治疗以及癌细胞的光学和荧光成像提供了一种创新方法。
