Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
J Colloid Interface Sci. 2017 Dec 15;508:323-331. doi: 10.1016/j.jcis.2017.08.050. Epub 2017 Aug 16.
In this paper, a redox-triggered drug delivery system of DOX/MSN-Au was prepared for chemo-photothermal synergistic therapy. The ultra-small gold nanoparticles (NPs) were appended to the openings of mesoporous silica nanoparticles (MSN) by Au-S bonds as the gatekeepers. Meanwhile, the gold NPs could be heated to high temperature by the near infrared (NIR) light irradiation, which is conducive to photothermal therapy. X-ray photoelectron spectroscopy (XPS) and Fourier Transform Infrared Spectrometer (FT-IR) spectra confirmed the formation of MSN-SH and MSN-Au. An in vitro NIR-induced photothermal study indicated that MSN-Au possessed concentration-dependent and power-dependent photothermal conversion capacity. Doxorubicin (DOX) was selected as the model drug loaded in the MSN. In vitro drug release showed that DOX released faster in the presence of glutathione (GSH) or NIR laser irradiation than without GSH or NIR irradiation, which suggested that the system had potentials for redox-responsive and NIR-triggered drug release. Confocal Laser Scanning Microscope (CLSM) was performed to evaluate the cellular uptake performance of DOX/MSN-Au. The cytotoxicity assay indicated that DOX/MSN-Au had a synergistic therapeutic effect by the combination of chemotherapy and photothermal therapy. This work suggested that MSN-Au could be explored as a redox-triggered drug delivery system for the chemo-photothermal synergistic therapy.
本文制备了一种 DOX/MSN-Au 的氧化还原触发药物递送系统，用于化学-光热协同治疗。超小的金纳米粒子（NPs）通过 Au-S 键连接到介孔硅纳米粒子（MSN）的开口处作为门控。同时，金 NPs 可以通过近红外（NIR）光照射被加热到高温，这有利于光热治疗。X 射线光电子能谱（XPS）和傅里叶变换红外光谱（FT-IR）谱证实了 MSN-SH 和 MSN-Au 的形成。体外近红外诱导光热研究表明，MSN-Au 具有浓度依赖性和功率依赖性的光热转换能力。阿霉素（DOX）被选为负载在 MSN 中的模型药物。体外药物释放实验表明，在有谷胱甘肽（GSH）或近红外激光照射的情况下，DOX 的释放速度比没有 GSH 或近红外照射的情况下更快，这表明该系统具有氧化还原响应和近红外触发药物释放的潜力。共聚焦激光扫描显微镜（CLSM）用于评估 DOX/MSN-Au 的细胞摄取性能。细胞毒性实验表明，DOX/MSN-Au 通过化疗和光热治疗的结合具有协同治疗效果。这项工作表明，MSN-Au 可以作为一种氧化还原触发的药物递送系统，用于化学-光热协同治疗。
