Pyun Do Gi, Yoon Hyoung Soon, Chung Ho Yun, Choi Hyun Jun, Thambi Thavasyappan, Kim Bong Sup, Lee Doo Sung
Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
J Mater Chem B. 2015 Oct 21;3(39):7752-7763. doi: 10.1039/c5tb00995b. Epub 2015 Sep 11.
Silver-containing dressings have been widely used to control wound infection. In this study, we developed various amounts of silver-hydroxyapatite (AgHAP)-containing polyurethane foams (PUFs) (AgHAP-PUFs), and their biological properties including biocompatibility, antibacterial activities, and in vivo wound healing properties were evaluated in the Sprague-Dawley rat model. From electron microscopy imaging, it was found that AgHAP particles are uniformly dispersed inside PUFs. The release of Ag from PUFs was dependent on both time and concentration, i.e., the amount of released Ag was significantly higher with increasing immersion time and Ag content in the PUFs. From the cytotoxicity test, AgHAP-PUFs exhibited high antibacterial efficacy against four pathogenic bacteria, and they were not cytotoxic against L-929 fibroblast cells. AgHAP-PUF treated groups exhibited scar-free wound healing by promoting re-epithelialization and collagen deposition in the infected excision wound model. Overall, it is evident that AgHAP-PUFs may be considered as a good antibacterial wound dressing for infected wounds due to their good antibacterial activity, biocompatibility, and wound healing rate.
含银敷料已被广泛用于控制伤口感染。在本研究中，我们制备了不同银羟基磷灰石（AgHAP）含量的聚氨酯泡沫（PUF）（AgHAP-PUF），并在Sprague-Dawley大鼠模型中评估了它们的生物学特性，包括生物相容性、抗菌活性和体内伤口愈合特性。从电子显微镜成像发现，AgHAP颗粒均匀分散在PUF内部。PUF中银的释放取决于时间和浓度，即随着浸泡时间的增加和PUF中银含量的增加，银的释放量显著更高。从细胞毒性试验来看，AgHAP-PUF对四种病原菌表现出高抗菌效果，并且对L-929成纤维细胞无细胞毒性。在感染切除伤口模型中，AgHAP-PUF处理组通过促进再上皮化和胶原蛋白沉积实现了无瘢痕伤口愈合。总体而言，由于其良好的抗菌活性、生物相容性和伤口愈合率，AgHAP-PUF可被视为感染伤口的良好抗菌伤口敷料。
