Zhao Hong, Lin Zhi Yuan, Yildirimer Lara, Dhinakar Arvind, Zhao Xin, Wu Jun
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
J Mater Chem B. 2016 Jun 21;4(23):4060-4071. doi: 10.1039/c6tb00308g. Epub 2016 May 23.
Therapeutic proteins have attracted significant attention as they perform vital roles in various biological processes. The delivery of therapeutic proteins to target sites is, however, challenging due to their intrinsic sensitivity to different environmental conditions. Polymeric nanoparticles (NPs) can offer not only physical protection from environmental stimuli but also targeted delivery of such proteins to specific sites. In particular, NPs containing charged polymers are preferred for many applications as they provide gentle protection through electrostatic interactions. Moreover, most organs exhibit a specific pH, and by tuning the extent of the electrostatic interactions and contact duration between the target organ and polymeric NPs, the intracellular uptake of the latter and thus long-term therapeutic efficacy can be optimized. In this article, we will critically discuss the design considerations of charged polymeric NPs, strategies for and routes of protein delivery and how these are influenced depending on the choice of delivery route.
治疗性蛋白质因其在各种生物过程中发挥着至关重要的作用而备受关注。然而，由于治疗性蛋白质对不同环境条件具有内在敏感性，将其递送至靶位点具有挑战性。聚合物纳米颗粒（NPs）不仅可以提供针对环境刺激的物理保护，还能将此类蛋白质靶向递送至特定位点。特别是，含有带电聚合物的纳米颗粒在许多应用中更受青睐，因为它们通过静电相互作用提供温和的保护。此外，大多数器官都具有特定的pH值，通过调节靶器官与聚合物纳米颗粒之间静电相互作用的程度和接触持续时间，可以优化后者的细胞内摄取，从而提高长期治疗效果。在本文中，我们将批判性地讨论带电聚合物纳米颗粒的设计考量、蛋白质递送的策略和途径，以及这些如何因递送途径的选择而受到影响。
